Calcium-triggered accumulation of defense-related transcripts enhances wheat resistance to leaf blast
Calcium-triggered accumulation of defense-related transcripts enhances wheat resistance to leaf blast
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DOI：
10.1007/s40858-017-0144-6
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发表时间：
2017-08-01
影响因子：
2.5
通讯作者：
Rodrigues, Fabricio A.
中科院分区：
文献类型：
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作者：
Debona, Daniel;Cruz, Maria F. A.;Rodrigues, Fabricio A.
钙（Ca）是植物生长所必需的大量营养元素，但其在植物抗病性中的作用尚不清楚。在这里，我们调查了钙是否增加防御相关转录本的丰度，并提高小麦对叶瘟的抗性，引起的Pyricularia pyramidae。为了解决这些问题，将小麦植物在含有三种Ca浓度（0.26、2.6和5 mM）的营养液中生长，所述营养液未接种或接种有P.随着营养液中Ca浓度从0.26增加到5 mM，叶片中Ca含量从1.3%增加到4.1%，稻瘟病严重度从36.8%降低到8.2%。（分别为病程相关1和丙二烯氧化物合酶）以及几丁质酶，β-1，3-葡聚糖酶，苯丙氨酸解氨酶，过氧化物酶和多酚氧化酶在5 mM Ca下生长的接种植物中比在0.26mM Ca下生长的接种植物中高。总的来说，结果表明，钙，除了其众所周知的作用，在细胞壁强化和抑制真菌分泌的果胶酶，在小麦叶瘟抗性起着积极的作用，通过上调防御相关基因的表达后，P. pseudo感染。SA和JA途径似乎构成了这种Ca介导的抗性的中心节点。
Calcium (Ca) is an essential macronutrient for plant growth, but its role in plant disease resistance remains largely elusive. Here, we investigated whether Ca increases the abundance of defense-related transcripts and enhances wheat resistance to leaf blast, caused by Pyricularia oryzae. To address these issues, wheat plants were grown in nutrient solution containing three Ca concentrations (0.26, 2.6 and 5 mM) either non-inoculated or inoculated with P. oryzae. Ca content in the leaves increased from 1.3 to 4.1% and blast severity decreased from 36.8 to 8.2% as the Ca concentration in the nutrient solution increased from 0.26 to 5 mM. The expression level of salicylic (SA) and jasmonic acid (JA) pathways gene markers (pathogenesis-related 1 and allene oxide synthase, respectively) as well as chitinase, beta-1,3-glucanase, phenylalanine ammonia lyase, peroxidase and polyphenol oxidase was higher in inoculated plants grown with 5 mM Ca than in those grown with 0.26 mM Ca. Collectively, results suggest that Ca, in addition to its well-known effect in cell wall strengthening and inhibition of fungal-secreted pectolytic enzymes, plays an active role in wheat resistance to leaf blast through upregulation of defense-related gene expression upon P. oryzae infection. Both of SA and JA pathways seems to make up a central node in such Ca-mediated resistance.