Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation
Development of a photoresponsive cell culture surface: Regional enhancement of living-cell adhesion induced by local light irradiation
复制标题
DOI：
10.1002/app.23197
复制
发表时间：
2006-04
影响因子：
3
通讯作者：
Y. Tada;K. Sumaru;Mitsuyoshi Kameda;Katsuhide Ohi;T. Takagi;T. Kanamori;Y. Yoshimi
中科院分区：
文献类型：
--
作者：
Y. Tada;K. Sumaru;Mitsuyoshi Kameda;Katsuhide Ohi;T. Takagi;T. Kanamori;Y. Yoshimi
利用含有硝基螺苯并吡喃残基和聚乙二醇的功能聚合物，我们研制了一种光响应型细胞培养表面(PRCS)，在该表面上，即使在活细胞存在的区域，也可以通过紫外光(350-400 nm，35 mW/cm2，5min)局部增强细胞粘附性。将接种于PRCS上的BALB/3T3成纤维细胞接种于PRCS上，用紫外线局部照射，培养12h后洗涤，去除几乎不贴壁的细胞，观察细胞黏附情况。结果表明，照射区的剩余细胞数最多是非照射区的2.5倍，这意味着一种新的方案的实现，即通过平行和同时的光照射来单独操纵活细胞。系统地研究了组成基片表面的聚合物材料的组成对其性能的影响。©2006威利期刊公司应用理工学院学报100：495-499,2006
Using a functional polymer containing nitrospirobenzopyran residues and poly(ethylene glycol), we developed a photoresponsive cell culture surface (PRCS), at which the cell adhesion can be enhanced locally by UV light irradiation (350–400 nm, 35 mW/cm2, 5 min) even on the region where living cells exist. Cell adhesion was evaluated by observing BALB/3T3 fibroblasts, which had been seeded on PRCS and then irradiated with UV light regionally, after 12 h incubation and subsequent washing to remove scarcely adhering cells. As a result, it was confirmed that the number of remaining cells in irradiated region was at most 2.5 times greater than that in nonirradiated regions, suggesting the implementation of a novel scheme to manipulate living cells individually by light irradiation in a parallel and simultaneous manner. Influence of the composition of the polymer material consisting the substrate surface was also investigated systematically. © 2006 Wiley Periodicals, Inc. J Appl Polym Sci 100: 495–499, 2006