Development of universal primers for isolating fragments of the LEAFY gene.
Development of universal primers for isolating fragments of the LEAFY gene.
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开发用于分离 LEAFY 基因片段的通用引物。
DOI：
10.4238/2013.june.6.1
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
B. Yan
中科院分区：
文献类型：
--
作者：
L. Yu;B. Yan
在所有陆地植物中都发现了叶状同源物。设计了简并引物用于扩增绿叶基因外显子3区片段。结果表明，该引物对所有陆生植物都具有普遍适用性。利用该方法，有可能利用绿叶基因外显子3作为单拷贝核标记，在高分类水平上解决网状进化问题，并阐明绿叶基因的起源和进化史。
LEAFY orthologs are found throughout land plants. Degenerate primers were designed for amplifying exon 3 region fragments of the LEAFY gene. Sixteen taxa from different plants were sampled, all of which were successfully isolated, which indicated that this set of primers is universally applicable for all land plants. With the use of this method, there may be potential for applying LEAFY exon 3 as a single-copy nuclear marker to resolve reticulate evolution at high taxonomic levels and to clarify the origin and evolutionary history of the LEAFY gene.