Daidzein Intake Is Associated with Equol Producing Status through an Increase in the Intestinal Bacteria Responsible for Equol Production
Daidzein Intake Is Associated with Equol Producing Status through an Increase in the Intestinal Bacteria Responsible for Equol Production
复制标题
DOI：
10.3390/nu11020433
复制
发表时间：
2019-02-01
期刊：
影响因子：
5.9
通讯作者：
Nakaji, Shigeyuki
中科院分区：
文献类型：
--
作者：
Iino, Chikara;Shimoyama, Tadashi;Nakaji, Shigeyuki
雌马酚是大豆苷元的代谢产物，对雌激素受体具有亲和力。虽然雌马酚是由肠道细菌产生的，但雌马酚产生状态与肠道微生物群之间的关联尚未得到充分研究。本研究的目的是比较在大豆苷元的摄入方面，在雌马酚生产者和非生产者受试者之间的肠道微生物群中负责雌马酚生产的肠道细菌。对参加广崎市健康调查的1044名成年人进行了检查。采用高效液相色谱法测定尿中雌马酚的浓度。使用16S rRNA扩增评估了肠道微生物群中负责雌马酚产生的8种细菌物种的相对丰度。有458名受试者被确定为雌马酚生产者。雌马酚产生状况和大豆苷元摄入量的比例随年龄增长而增加。雌马酚生产者大豆黄酮日摄入量较大。将大豆苷元转化为雌马酚的肠道细菌存在于雌马酚生产者和非生产者中。但是，雌马酚生产者中的Ascorrhocelatus和Slackia pecioniconconvertens的相对丰度和患病率显著高于非生产者。将大豆苷元转化为雌马酚的肠道细菌不仅存在于雌马酚生产者中，也存在于非生产者中。大豆黄酮的摄入量与雌马酚的产生状态有关，通过增加A。celatus和S.在肠道菌群中转化。
Equol is a metabolite of isoflavone daidzein and has an affinity to estrogen receptors. Although equol is produced by intestinal bacteria, the association between the status of equol production and the gut microbiota has not been fully investigated. The aim of this study was to compare the intestinal bacteria responsible for equol production in gut microbiota between equol producer and non-producer subjects regarding the intake of daidzein. A total of 1044 adult subjects who participated in a health survey in Hirosaki city were examined. The concentration of equol in urine was measured by high-performance liquid chromatography. The relative abundances of 8 bacterial species responsible for equol production in the gut microbiota was assessed using 16S rRNA amplification. There were 458 subjects identified as equol producers. The proportion of equol production status and the intake of daidzein increased with age. Daily intake of daidzein was larger in equol-producer. The intestinal bacteria, which convert daidzein to equol were present in both equol producers and non-producers. However, the relative abundance and the prevalence of Asaccharobacter celatus and Slackia isoflavoniconvertens were significantly higher in equol producers than those in equol non-producers. The intestinal bacteria that convert daidzein to equol are present in not only the equol producers but also in the non-producers. The daidzein intake is associated with the equol production status through an increase of A. celatus and S. isoflavoniconvertens in the gut microbiota.