Isolation and characterization of 40 polymorphic microsatellite markers from Parambassis siamensis
Isolation and characterization of 40 polymorphic microsatellite markers from Parambassis siamensis
复制标题
暹罗副鲈 40 个多态性微卫星标记的分离和鉴定
DOI：
10.1007/s12686-012-9700-z
复制
发表时间：
2012
影响因子：
1.1
通讯作者：
T. W.(他5名)
中科院分区：
文献类型：
--
作者：
Koizumi;N.;Morioka;S.;Quinn;T. W.(他5名)
我们从Parambassis siamensis 基因组中分离和鉴定了总共40 个多态性微卫星位点。开发了侧翼聚合酶链反应引物并用于显示所有基因座都是多态性的，在 32 个个体中每个基因座观察到的等位基因数量为 2 至 29 个。观察到的杂合度范围为 0.063 至 0.969，而预期杂合度范围为 0.062 至 0.960。所有基因座均符合 Hardy-Weinberg 平衡，没有一个基因座组合显示出显着的连锁不平衡，并且没有基因座显示无效等位基因的证据。这 40 个微卫星位点对于未来研究 P.P. 种群的遗传分化和结构应该有用。暹罗。
We isolated and characterized a total of 40 polymorphic microsatellite loci from the genome ofParambassis siamensis. Flanking Polymerase Chain Reaction primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 32 individuals ranging from 2 to 29. The observed heterozygosity ranged from 0.063 to 0.969, while the expected heterozygosity varied from 0.062 to 0.960. All loci conformed to Hardy–Weinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 40 microsatellite loci should be useful for conducting future investigations into the genetic differentiation and structure of populations ofP. siamensis.