Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869
Impact of mycolic acid deficiency on cells of Corynebacterium glutamicum ATCC13869
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分枝菌酸缺乏对谷氨酸棒杆菌ATCC13869细胞的影响
DOI：
10.1002/bab.1622
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发表时间：
2018-05-01
影响因子：
2.8
通讯作者：
Wang, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Gao, Yunfei;Hu, Xiaoqing;Wang, Xiaoyuan
Mycolic acid (MA) plays important role in Corynebacterium glutamicum, but the key enzymes in the biosynthetic pathway of MA in C. glutamicum ATCC13869 have not been characterized. Since the locus BBD29_RS14045 in C. glutamicum ATCC13869 shows high similarity to the gene Cgl2871, which encodes Pks13, the key enzyme for synthesizing MA in C. glutamicum ATCC13032, it was deleted, resulting in the mutant WG001. Compared with the wild‐type ATCC13869, MA was not synthesized in WG001, but more phosphatidylglycerol and phosphatidylinositol containing longer unsaturated fatty acids were produced. WG001 cells also show hindered cell growth and defective cell separation when compared with ATCC13869 cells. Transcriptomic analysis shows that many genes relevant to the pathways of fatty acids, inositol, phospholipids, cell wall, and cell division were significantly regulated in WG001 cells when compared with ATCC13869 cells. This study demonstrates that the locus BBD29_RS14045 encodes a key enzyme that plays important role for synthesizing MA in C. glutamicum ATCC13869.
