In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.
In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.
复制标题
用于研究轴突引导中 PCP 信号传导的体外外植体测定和培养。
DOI:
10.1007/978-1-0716-2035-9_18
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发表时间:
2022
期刊:
影响因子:
--
通讯作者:
Zou,Yimin
中科院分区:
文献类型:
--
作者:
Marfull-Oromí,Pau;Miller,KathrynM;Zou,Yimin
In vitro studies have provided valuable insights to the function and mechanisms in axon guidance. In this chapter, we will introduce the rodent “open-book” assay, pre- or postcrossing explant culture and the dissociated neuron culture. They have been used to discover mechanism which we have gone on to validate or will confirm using in vivo genetic approaches.