In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.

In Vitro Explant Assays and Cultures to Study PCP Signaling in Axon Guidance.
复制标题

用于研究轴突引导中 PCP 信号传导的体外外植体测定和培养。

DOI:
10.1007/978-1-0716-2035-9_18
复制
发表时间:
2022
期刊:
Methods in molecular biology (Clifton, N.J.)
影响因子:
--
通讯作者:
Zou,Yimin
Zou,Yimin
中科院分区:
--
文献类型:
--
作者:
Marfull-Oromí,Pau;Miller,KathrynM;Zou,Yimin

文献摘要

相似文献

体外研究为轴突导向的功能和机制提供了有价值的见解。在本章中,我们将介绍啮齿类动物的“开卷”试验、杂交前后的外植体培养和分离的神经元培养。它们已被用于发现机制,我们已经继续验证或将使用体内遗传方法确认。
In vitro studies have provided valuable insights to the function and mechanisms in axon guidance. In this chapter, we will introduce the rodent “open-book” assay, pre- or postcrossing explant culture and the dissociated neuron culture. They have been used to discover mechanism which we have gone on to validate or will confirm using in vivo genetic approaches.