Digital adaptive optics line-scanning confocal imaging system.
Digital adaptive optics line-scanning confocal imaging system.
复制标题
数字自适应光学线扫描共焦成像系统。
DOI：
10.1117/1.jbo.20.11.111203
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发表时间：
2015
影响因子：
3.5
通讯作者：
Kim,MyungK
中科院分区：
文献类型：
--
作者：
Liu,Changgeng;Kim,MyungK
将数字全息自适应光学技术应用于数字形式的线扫描共焦成像系统，提出了一种数字自适应光学线扫描共焦成像系统。在DAOLCI中，每一行扫描都由数字全息图记录，这允许通过数字全息术从样品的一个切片访问复杂的光学场。这个复杂的光场包含了样品的一个切片的信息和系统的光学像差，从而使我们能够补偿光学像差的影响，这可以通过一个复杂的导星全息图来感知。经过数值像差补偿后，将一系列线扫描的校正光场拼接成最终的校正共焦像。在DAOLCI中，采用数值狭缝来实现传感器端的共聚焦。狭缝的宽度可以调节，以控制散射样品的图像对比度和散斑噪声。DAOLCI省去了传统自适应光学共聚焦成像系统中采用的Shack-Hartmann波前传感器、变形镜等硬件部件和闭环反馈，降低了光学机械复杂性和成本。数值模拟和原理验证实验证明了该方法的可行性。
A digital adaptive optics line-scanning confocal imaging (DAOLCI) system is proposed by applying digital holographic adaptive optics to a digital form of line-scanning confocal imaging system. In DAOLCI, each line scan is recorded by a digital hologram, which allows access to the complex optical field from one slice of the sample through digital holography. This complex optical field contains both the information of one slice of the sample and the optical aberration of the system, thus allowing us to compensate for the effect of the optical aberration, which can be sensed by a complex guide star hologram. After numerical aberration compensation, the corrected optical fields of a sequence of line scans are stitched into the final corrected confocal image. In DAOLCI, a numerical slit is applied to realize the confocality at the sensor end. The width of this slit can be adjusted to control the image contrast and speckle noise for scattering samples. DAOLCI dispenses with the hardware pieces, such as Shack–Hartmann wavefront sensor and deformable mirror, and the closed-loop feedbacks adopted in the conventional adaptive optics confocal imaging system, thus reducing the optomechanical complexity and cost. Numerical simulations and proof-of-principle experiments are presented that demonstrate the feasibility of this idea.