Initial Studies on the Regulation of Toluene Degradation by Pseudomonas Putida F1
Initial Studies on the Regulation of Toluene Degradation by Pseudomonas Putida F1
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恶臭假单胞菌F1降解甲苯调控的初步研究
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
D. Gibson
中科院分区：
文献类型：
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作者：
B. Finette;D. Gibson
恶臭假单胞菌 Fl 通过顺式甲苯二氢二醇将甲苯氧化为 3-甲基儿茶酚。后一种化合物是芳核“间”裂变的底物。动力学和诱导实验表明，编码这些反应的酶的基因是操纵子的一部分，称为 tod 操纵子，它是协调诱导和调节的。通过使用氧化还原染料 2,3,5-三苯基-2H-氯化四唑的筛选程序，以高频率分离出无法利用甲苯作为生长底物的菌株。 tod操纵子结构基因突变菌株的生化特征表明，甲苯诱导恶臭假单胞菌Fl降解甲苯中的前四种酶。多效性阴性突变体以及 tod 基因表达发生改变的突变体的分离和表征表明，tod 操纵子可能处于正调控元件的控制之下。
Pseudomonas putida Fl oxidizes toluene through cis-toluene dihydrodiol to 3-methylcatechol. The latter compound is the substrate for “meta” fission of the aromatic nucleus. Kinetic and induction experiments indicate that the genes encoding enzymes for these reactions are part of an operon, designated the tod operon, that is coordinately induced and regulated. Strains unable to utilize toluene as a growth substrate were isolated at high frequencies by using screening procedures that utilize the redox dye, 2,3,5-triphenyl-2H-tetrazolium chloride. Biochemical characterization of strains with mutations in the structural genes of the tod operon showed that toluene induces the first four enzymes in toluene degradation by P. putida Fl. The isolation and characterization of pleiotropicnegative mutants together with mutants altered in terms of their expression of tod genes suggests that the tod operon may be under the control of a positive regulatory element.