Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response
Mycobacterium tuberculosis sigma factor E regulon modulates the host inflammatory response
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DOI：
10.1086/591098
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发表时间：
2008-09-15
影响因子：
6.4
通讯作者：
Smith, Issar
中科院分区：
文献类型：
--
作者：
Fontan, Patricia A.;Aris, Virginie;Smith, Issar
结核分枝杆菌在巨噬细胞中存活，通常破坏这些巨噬细胞的杀菌机制。了解这种宿主-病原体相互作用对开发新的结核病治疗方法具有重要意义。结核分枝杆菌对细胞内生活的适应取决于其调节其基因表达的能力。Sigma因子是重要的细菌转录激活因子，它与RNA聚合酶结合并赋予其启动子特异性。西格玛因子E （SigE）控制对毒力至关重要的基因的表达。我们确定了巨噬细胞感染过程中的SigE调控子，并分析了该调控子对吞噬细胞转录反应的影响。我们的研究结果表明，SigE调节巨噬细胞感染期间参与维持结核分枝杆菌包膜完整性和功能的基因的表达。对吞噬细胞转录反应的分析表明SigE调节因子参与了炎症反应的调节。
Mycobacterium tuberculosis survives in macrophages and usually subverts the bactericidal mechanisms of these phagocytes. The understanding of this hostpathogen interaction is relevant for the development of new treatments for tuberculosis. The adaptation of M. tuberculosis to intracellular life depends on its ability to regulate the expression of its genes. Sigma factors are important bacterial transcription activators that bind to the RNA polymerase and give it promoter specificity. Sigma factor E (SigE) controls the expression of genes that are essential for virulence. We have identified the SigE regulon during infection of macrophages, and we analyzed the impact of this regulon on the transcriptional response of phagocytes. Our results indicate that SigE regulates the expression of genes involved in the maintenance of M. tuberculosis cell envelope integrity and function during macrophage infection. Analysis of the phagocytes' transcriptional response indicates that the SigE regulon is involved in the modulation of the inflammatory response.