Antioxidative Activity of a Zinc-Chelating Substance in Coffee
Antioxidative Activity of a Zinc-Chelating Substance in Coffee
复制标题
咖啡中锌螯合物质的抗氧化活性
DOI：
10.1271/bbb.68.2313
复制
发表时间：
2004
期刊：
影响因子：
--
通讯作者：
S. Homma
中科院分区：
文献类型：
--
作者：
X. Wen;M. Takenaka;M. Murata;S. Homma
咖啡含有一种棕色的锌螯合聚合物，称为ApV。通过添加ZnCl2从速溶咖啡溶液中形成的沉淀制备ApV，并使用离子交换和纤维素柱色谱法纯化。本研究对ApV及其相关化合物的抗氧化活性进行了评价。通过ABTS测定法估计的ApV的自由基清除活性与速溶咖啡的水平相似，而ApV的O2−清除活性（超氧化物歧化酶样活性）低于速溶咖啡。ApV对羟自由基的清除活性高于速溶咖啡，对亚油酸自氧化的抑制作用强于咖啡酸。
Coffee brew contains a brownish zinc-chelating polymer designated ApV. ApV was prepared from the precipitate formed in a solution of instant coffee by adding ZnCl2 and purified using ion-exchange and cellulose column chromatographies. The antioxidative activities of ApV and related compounds were evaluated in this study. The free-radical scavenging activity of ApV estimated by ABTS assay was at a similar level to that of instant coffee, while the O2− scavenging activity of ApV, which is superoxide dismutase-like activity, was lower than that of instant coffee. The hydroxyl-radical scavenging activity of ApV was higher than that of instant coffee, and the auto-oxidation of linoleic acid was more strongly inhibited by ApV than by caffeic acid.