Studies on murine thyroiditis: new insights from organ flow cytometry.
Studies on murine thyroiditis: new insights from organ flow cytometry.
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小鼠甲状腺炎研究：器官流式细胞术的新见解。
DOI：
10.1089/105072503322021070
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Tzou,Shey-Cherng
中科院分区：
文献类型：
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作者：
Caturegli,Patrizio;Rose,NoelR;Kimura,Miho;Kimura,Hiroaki;Tzou,Shey-Cherng
自身免疫性疾病的发病机制经常在小鼠模型中进行研究，其中疾病结果传统上是通过光学显微镜评估的。为了确定数字成像是否提高了组织病理学评估的可靠性，以及流式细胞术是否直接适用于小鼠甲状腺，我们研究了395只免疫甲状腺球蛋白3周的CBA/J小鼠和192只未免疫的CBA/J小鼠。数字成像显著提高了组织病理学评估的可靠性（r= 0.988, 95%可信区间：0.980-0.992,p< 0.0001），可以成功地对小鼠甲状腺进行流式细胞术。我们还发现正常甲状腺间质中含有比预期数量更多的造血细胞。我们认为数字成像提供了一种更好的估计疾病结果的手段，并且在靶器官水平上进行的流式细胞术比在外周淋巴器官上进行的流式细胞术更能反映自身免疫发病机制。这些方法也适用于自身免疫攻击的其他器官系统，如心脏、外分泌和其他内分泌腺体。
The pathogenesis of autoimmune diseases is frequently studied in murine models, in which disease outcome is traditionally assessed by light microscopy. To determine whether digital imaging improves reliability of the histopathologic assessment, and whether flow cytometry is applicable directly on the murine thyroid, we studied 395 CBA/J mice 3 weeks after thyroglobulin immunization, and 192 nonimmunized CBA/J mice. Digital imaging significantly improved reliability of the histopathological assessment (r= 0.988, 95% confidence interval: 0.980-0.992,p< 0.0001), and flow cytometry on the murine thyroid could be performed successfully. We also found that normal thyroids contained a higher than expected number of hematopoietic cells in the interstitium. We suggest that digital imaging offers a better means of estimating disease outcome, and that flow cytometry performed at the target organ levels reflects the autoimmune pathogenesis more closely than when performed on peripheral lymphoid organs. These methods should also be applicable to other organ systems targeted by autoimmune attack, such as heart, exocrine, and other endocrine glands.