Efficacy of silver nanoparticles against the adults and eggs of monogenean parasites of fish
Efficacy of silver nanoparticles against the adults and eggs of monogenean parasites of fish
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DOI：
10.1007/s00436-019-06315-9
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发表时间：
2019-06-01
影响因子：
2
通讯作者：
Josefina Fajer-Avila, Emma
中科院分区：
文献类型：
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作者：
Altagracia Pimentel-Acosta, Citlalic;Neptali Morales-Serna, Francisco;Josefina Fajer-Avila, Emma
单殖吸虫是一种常见于鱼类身上的多种寄生虫。一些单殖物种对养殖鱼类具有高致病性。本研究旨在利用 Cichlidogyrus spp 确定银纳米颗粒 (AgNP) 对淡水中单殖纲成虫和卵的体外驱虫作用。作为模式生物。我们测试了两种具有不同合成方法和尺寸直径的 AgNP：ARGOVIT (35 nm) 和 UTSA (1-3 nm) 纳米颗粒。通过扫描电子显微镜观察寄生虫外皮的损伤。 UTSA AgNPs 比 ARGOVIT 更有效；在这两种情况下，都存在浓度依赖性效应。浓度为 36g/L 的 UTSA AgNP 持续 1 小时，对虫卵和成虫寄生虫具有 100% 的效果，会导致寄生虫肿胀、波纹消失和外皮破坏。考虑到单殖卵通常对抗寄生虫药物和化学制剂具有耐受性，这是一个有趣的结果。据我们所知，之前没有任何报告评估过纳米银颗粒对鱼类后生动物寄生虫的影响。因此，本研究为今后鱼类寄生虫病防治研究奠定了基础。
Monogeneans are a diverse group of parasites that are commonly found on fish. Some monogenean species are highly pathogenic to cultured fish. The present study aimed to determine the in vitro anthelmintic effect of silver nanoparticles (AgNPs) against adults and eggs of monogeneans in freshwater using Cichlidogyrus spp. as a model organism. We tested two types of AgNPs with different synthesis methodologies and size diameters: ARGOVIT (35nm) and UTSA (1-3nm) nanoparticles. Damage to the parasite tegument was observed by scanning electron microscopy. UTSA AgNPs were more effective than ARGOVIT; in both cases, there was a concentration-dependent effect. A concentration of 36g/L UTSA AgNPs for 1h was 100% effective against eggs and adult parasites, causing swelling, loss of corrugations, and disruption of the parasite's tegument. This is an interesting result considering that monogenean eggs are typically tolerant to antiparasite drugs and chemical agents. To the best of our knowledge, no previous reports have assessed the effect of AgNPs on any metazoan parasites of fish. Therefore, the present work provides a basis for future research on the control of fish parasite diseases.