Utilization of potato starch processing wastes to produce animal feed with high lysine content.
Utilization of potato starch processing wastes to produce animal feed with high lysine content.
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DOI：
10.4014/jmb.1404.04035
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发表时间：
2015-02
影响因子：
2.8
通讯作者：
Ying Li;Bingnan Liu;Jinzhu Song;Cheng Jiang;Qian Yang
中科院分区：
文献类型：
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作者：
Ying Li;Bingnan Liu;Jinzhu Song;Cheng Jiang;Qian Yang
本研究旨在利用马铃薯淀粉工业的废弃物生产高赖氨酸含量的单细胞蛋白（SCP）作为动物饲料。本研究利用抗S-（2-氨基乙基）- l -半胱氨酸的短小芽孢杆菌E1生产赖氨酸含量高的SCP，利用黑曲霉降解纤维素生物量，利用假丝酵母改善饲料的气味和口感。采用正交设计优化发酵工艺，以获得最大赖氨酸含量。最佳发酵条件为：温度40℃，底物浓度3%，自然pH 7.0左右。对于未灭菌的马铃薯淀粉废弃物，通过细菌16S rRNA基因末端限制性内切片段长度多态性分析确定发酵过程中的微生物群落。结果表明，菌株的优势菌群为芽孢杆菌，第0天的产物蛋白质质量和氨基酸谱均显著高于未处理的产物。急性毒性试验表明SCP产品无毒，可用于商业加工。
This work aims to utilize wastes from the potato starch industry to produce single-cell protein (SCP) with high lysine content as animal feed. In this work, S-(2-aminoethyl)-L-cysteine hydrochloride-resistant Bacillus pumilus E1 was used to produce SCP with high lysine content, whereas Aspergillus niger was used to degrade cellulose biomass and Candida utilis was used to improve the smell and palatability of the feed. An orthogonal design was used to optimize the process of fermentation for maximal lysine content. The optimum fermentation conditions were as follows: temperature of 40°C, substrate concentration of 3%, and natural pH of about 7.0. For unsterilized potato starch wastes, the microbial communities in the fermentation process were determined by terminal restriction fragment length polymorphism analysis of bacterial 16S rRNA genes. Results showed that the dominant population was Bacillus sp. The protein quality as well as the amino acid profile of the final product was found to be significantly higher compared with the untreated waste product at day 0. Additionally, acute toxicity test showed that the SCP product was non-toxic, indicating that it can be used for commercial processing.