Herbicide propisochlor exposure induces intestinal barrier impairment, microbiota dysbiosis and gut pyroptosis.
Herbicide propisochlor exposure induces intestinal barrier impairment, microbiota dysbiosis and gut pyroptosis.
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DOI：
10.1016/j.ecoenv.2023.115154
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发表时间：
2023-06
影响因子：
6.8
通讯作者：
Yunle Liu;Lin Ye;Huodai Chen;K. Tsim;Xing Shen;Xiangmei Li;Xueling Li;Hongtao Lei
中科院分区：
文献类型：
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作者：
Yunle Liu;Lin Ye;Huodai Chen;K. Tsim;Xing Shen;Xiangmei Li;Xueling Li;Hongtao Lei
异丙草胺是一种氯乙酰胺类除草剂，对人和动物有肝毒性和免疫抑制作用。尽管这种除草剂已经使用多年，但异丙草胺对肠道健康的影响仍然知之甚少。因此，异丙草胺对肠道健康和肠道微生物群的影响通过使用分子方法和细菌16S rRNA测序进行分析。结果表明，异丙草胺可引起小鼠肠道形态学改变，紧密连接蛋白表达减少，粘液层厚度降低，并激活了细胞凋亡信号。此外，异丙草胺在小鼠体内的暴露导致肠道微生物多样性和组成的显著改变，包括拟杆菌门的增加和厚壁菌门的减少。肠道微生物群中的类杆菌、类梭菌和类杆菌与肠道健康呈强负相关。这些结果表明，肠道菌群可能在异丙草胺诱导的焦亡中发挥关键作用。
Propisochlor is a chloroacetamide herbicide causing liver toxicity and suppressing immunity in human and animal. Although the herbicide has been used for years, the effects of propisochlor on intestinal health remain poorly understood. Hence, the impacts of propisochlor in intestinal health and gut microbiota were analyzed by using molecular approach and bacterial 16S rRNA sequencing. The result showed that the intake of propisochlor in mice impaired gut morphology, reduced expression of tight junction proteins, decreased thickness of mucus layer and activated pyroptosis signaling. Moreover, the exposure of propisochlor in mice led to significant alterations in gut microbial diversity and composition, including an increase of Bacteroidetes and a decrease of Firmicutes. The gut microbiota, such asParabacteroides,Parasutterella, andBacteroides, demonstrated a strong negative correlation with the intestinal health. These findings suggested that gut microbiota could play a critical role in the propisochlor-induced pyroptosis.