In vitro effects of vomitoxin (deoxynivalenol) on T-cell interleukin production and IgA secretion.
In vitro effects of vomitoxin (deoxynivalenol) on T-cell interleukin production and IgA secretion.
复制标题
呕吐毒素（脱氧雪腐镰刀菌烯醇）对 T 细胞白细胞介素生成和 IgA 分泌的体外影响。
DOI：
10.1016/0278-6915(94)90005-1
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Pestka,JJ
中科院分区：
文献类型：
--
作者：
Warner,RL;Brooks,K;Pestka,JJ
淋巴细胞培养物暴露于呕吐毒素，以确定可能的机制，这种天然存在的毒素诱导血清免疫球蛋白A（伊加）的升高和伊加肾病的小鼠。在10至1000 ng/ml范围内的呕吐毒素暴露抑制DNA合成，蛋白质合成以及伊加，IgG和IgM的生产从派尔集合淋巴结（PP）和脾脏制备的淋巴细胞培养物。当在呕吐毒素存在下培养纯化的B细胞时，类似地观察到伊加、IgG和IgM产生的抑制。然而，在24小时脉冲共暴露于呕吐毒素和促分裂原伴刀豆球蛋白A（ConA），CD 4 +/CD 8+细胞能够诱导3 - 5倍的伊加的生产增加，但不是IgG和IgM的共培养的B细胞相比，与对照组接触到促分裂原的T细胞共培养的B细胞。当用ConA和毒素脉冲48小时时，CD 4+细胞同样能够引起B细胞伊加产生的显著增加。与单独ConA刺激的CD 4+细胞相比，48小时脉冲暴露于ConA和呕吐毒素导致T辅助细胞因子IL-4、IL-5和IL-6的产生在另外5天的培养后显著增加。这些结果表明，呕吐毒素能够增强CD 4+介导的帮助B细胞产生伊加，这可能是通过增加细胞因子的产生来介导的。
The exposure of lymphocyte cultures to vomitoxin was used to determine possible mechanisms by which this naturally occurring toxin induces serum immunoglobulin A (IgA) elevation and IgA nephropathy in the mouse. Vomitoxin exposure within the range of 10 to 1000 ng/ml inhibited DNA synthesis, protein synthesis as well as IgA, IgG and IgM production in lymphocyte cultures prepared from the Peyer's patch (PP) and spleen. When purified B cells were cultured in the presence of vomitoxin, inhibition of IgA, IgG and IgM production was similarly observed. However, on 24-hr pulsed co-exposure to vomitoxin and the mitogen concanavalin A (ConA), CD4+/CD8+cells were capable of inducing a three- to five-fold increase in production of IgA, but not IgG and IgM by cocultured B cells when compared with B cells cocultured with control T cells exposed to the mitogen only. When pulsed for 48 hr with ConA and toxin, CD4+cells were similarly capable of causing a significant increase in IgA production by B cells. 48-hr pulsed exposure of CD4+cells to ConA and vomitoxin resulted in significantly increased production of the T helper cytokines IL-4, IL-5 and IL-6 after 5 additional days of culture, compared with ConA-stimulated CD4+cells alone. These results suggest that vomitoxin was capable of enhancing CD4+-mediated help for IgA production by B cells and that this could possibly be mediated by way of increased cytokine production.