Detection and Molecular Characterization of Aichivirus 1 in Wastewater Samples from Uruguay
Detection and Molecular Characterization of Aichivirus 1 in Wastewater Samples from Uruguay
复制标题
DOI：
10.1007/s12560-015-9217-1
复制
发表时间：
2016-03-01
影响因子：
3.4
通讯作者：
Victoria, M.
中科院分区：
文献类型：
--
作者：
Burutaran, L.;Lizasoain, A.;Victoria, M.
艾奇病毒1（AiV-1）是一种直径为30 nm的肠道病毒，属于小核糖核酸病毒科科中的科布病毒属，是人类胃肠炎的病原体。传播途径包括粪口途径、人与人接触、在受污染的沃茨娱乐或食用受污染的食物或水。本研究的目的是确定从乌拉圭的废水中的频率和AiV-1的分子特征。2011年3月至2012年2月，每两周一次的音乐会在乌拉圭西北部地区的贝拉联盟、萨尔托、佩桑杜和弗雷·本托斯等城市举行。共收集了96份样品;通过超离心浓缩病毒，并通过使用针对病毒基因组保守区域（3CD连接）的引物的巢式PCR检测AIV-1。AIV-1（n = 54）的高频率，观察到在所有的城市分析，主要是在寒冷的月份的一年。AiV-1未被证明是适当的病毒粪便指示剂，因为与先前检测到的其他肠道病毒相比，未观察到相关性。利用第一轮PCR扩增产物进行系统发育分析，结果表明13株AIV-1均属于基因型B。这项研究表明，AIV-1是乌拉圭西北部地区常见的肠道病毒，存在于废水中，由感染者排出。
Aichivirus 1 (AiV-1) is an enteric virus with 30 nm in diameter, belonging to the genus Kobuvirus in the Picornaviridae family being a causative agent of gastroenteritis in humans. The transmission is via the fecaloral route, through person to person contact, recreation in contaminated waters, or through the consumption of contaminated food or water. The aim of this study was to determine the frequency and the molecular characterization of AiV-1 in wastewater from Uruguay. Biweekly collections from March 2011 to February 2012 were performed in the cities of Bella Union, Salto, Paysandu, and Fray Bentos, northwestern region of Uruguay. A total of 96 samples were collected; viruses were concentrated by ultracentrifugation, and AiV-1 was detected by using a nested PCR with primers directed to a conserved region (3CD junction) of the viral genome. A high frequency of AiV-1 (n = 54) was observed at all the cities analyzed mainly in the colder months of the year. AiV-1 was not evidenced as an appropriate viral fecal indicator since when compared with other previously detected enteric viruses, no correlation was observed. All 13 characterized AiV-1 belonged to the genotype B after the phylogenetic analysis performed with the sequences obtained from the first round PCR amplicon. This study demonstrates that AiV-1 is a frequently detected enteric viruses present in wastewater and excreted by infected persons in the northwestern region of Uruguay.