Membrane fouling by cell‐protein mixtures: In situ characterisation using multi‐photon microscopy
Membrane fouling by cell‐protein mixtures: In situ characterisation using multi‐photon microscopy
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细胞蛋白质混合物造成的膜污染：使用多光子显微镜进行原位表征
DOI：
10.1002/bit.21113
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发表时间：
2007
影响因子：
3.8
通讯作者：
U. Tirlapur
中科院分区：
文献类型：
--
作者：
D. Hughes;Z. Cui;R. Field;U. Tirlapur
细胞和蛋白质混合物对膜的污染会导致通量严重下降，导致最终需要清洁或更换膜。在这项研究中，利用多光子显微镜，一种基于荧光的技术，对含有洗涤酵母、牛血清白蛋白（BSA）和卵清蛋白组合的悬浮液对微滤膜的污染进行了三维原位成像。适当的荧光标记可以清楚地识别三种污染物。图像与过滤数据关联良好，并清楚地显示酵母细胞饼捕获蛋白质聚集体。蛋白质表现出非常不同的过滤行为。当与卵白蛋白和/或BSA和卵白蛋白质量为50:50的混合物一起过滤水洗酵母时，卵白蛋白污染主导了系统。饼捕获聚集体并没有减少蛋白质对膜的污染，反而增加了饼的阻力。对于牛血清白蛋白和水洗酵母的混合物，酵母细胞饼的存在确实减少了蛋白质对膜的污染，然而，由于饼的额外阻力导致通量低于单独过滤牛血清白蛋白时的通量。Biotechnol。Bioeng。2007;96:1083 - 1091。©2006 Wiley期刊公司
Fouling of the membrane by cell and protein mixtures can result in severe flux declines, leading to the eventual need to clean or replace the membrane. In this study multi‐photon microscopy, a fluorescence‐based technique is used to 3‐D image in situ the fouling of microfiltration membranes by suspensions containing combinations of washed yeast, bovine serum albumin (BSA) and ovalbumin. Appropriate fluorescent labelling allows the three foulant species to be clearly identified. Images correlate well with filtration data and clearly show the cake of yeast cells capturing protein aggregates. The proteins exhibited very different filtration behaviour. When filtering washed yeast together with ovalbumin and/or a 50:50 mixture by mass of BSA and ovalbumin, the ovalbumin fouling dominates the system. Capture of aggregates by the cake did not reduce fouling of the membrane by the protein and increased the resistance of the cake. For mixtures of BSA and washed yeast, the presence of a cake of yeast cells did reduce fouling of the membrane by the protein, however, the extra resistance due to the cake resulted in a flux lower than that when filtering BSA alone. Biotechnol. Bioeng. 2007;96:1083–1091. © 2006 Wiley Periodicals, Inc.