Potential of Induced Pluripotent Stem Cells for the Regeneration of the Tracheal Wall
Potential of Induced Pluripotent Stem Cells for the Regeneration of the Tracheal Wall
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DOI：
10.1177/000348941011901009
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发表时间：
2010-10-01
影响因子：
1.4
通讯作者：
Omori, Koichi
中科院分区：
文献类型：
--
作者：
Imaizumi, Mitsuyoshi;Nomoto, Yukio;Omori, Koichi
目的：我们以往的研究主要集中在人工气管的基础研究和临床应用上。然而，预制的人工气管不能用于儿科呼吸道，因为随着儿童的发育，气管框架需要扩大。本研究旨在探讨诱导多能干细胞(IPS)在气管壁处再生的可能性。方法：在软骨细胞分化介质(生物工程支架模型)的三维支架中培养iPS细胞，并与不含iPS细胞的三维支架(对照支架模型)的培养结果进行比较。将3-D支架植入8只裸鼠的气管缺损处。结果：植入生物工程支架的5只大鼠体内均存在iPS细胞。在生物工程支架模型的5只大鼠中，有2只在再生的气管壁上观察到软骨样组织，而在对照支架模型的3只大鼠中没有观察到软骨样组织。气管缺损处有软骨样组织再生。本研究证实了iPS细胞在气管壁再生中的潜力。
Objectives: Our previous studies focused on basic research and the clinical applications of an artificial trachea. However, the prefabricated artificial trachea cannot be utilized for pediatric airways, because the tracheal frame needs to expand as the child develops. The purpose of this study was to evaluate the potential of induced pluripotent stem (iPS) cells for the regeneration of the tracheal wall.Methods: We cultured iPS cells in a 3-dimensional (3-D) scaffold in chondrocyte differentiation medium (bioengineered scaffold model), and the results were compared with those in a 3-D scaffold without iPS cells (control scaffold model). The 3-D scaffolds were implanted into tracheal defects in 8 nude rats. After 4 weeks, the regenerated tissue was histologically examined.Results: Implanted iPS cells were confirmed to exist in all 5 rats implanted with bioengineered scaffolds. Cartilage-like tissue was observed in the regenerated tracheal wall in 2 of the 5 rats in the bioengineered scaffold model, but in none of the 3 rats in the control scaffold model.Conclusions: Implanted iPS cells were confirmed to exist in the bioengineered scaffold. Cartilage-like tissue was regenerated in the tracheal defect. This study demonstrated the potential of iPS cells in the regeneration of the tracheal wall.