The removal of leukocytes and platelets from whole blood.
The removal of leukocytes and platelets from whole blood.
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DOI：
10.5555/uri:pii:0022214376903814
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发表时间：
1976-08
期刊：
影响因子：
--
通讯作者：
E. Beutler;C. West;K. Blume
中科院分区：
文献类型：
--
作者：
E. Beutler;C. West;K. Blume
对从全血中去除白细胞的各种方法进行了比较，并设计了一种新技术。该程序包括使全血通过由微晶纤维素和α-纤维素组成的床。该方法快速、可靠，可从血液中去除超过99.75%的白细胞，并且似乎不会选择性地保留网织红细胞或释放大量白细胞酶。大多数血小板也从抗凝血液中除去，通过使去血小板血液通过微晶纤维素-α-纤维素床可以获得无血小板红细胞。
Various methods for the removal of leukocytes from whole blood have been compared and a new technique has been devised. This procedure consists of passing whole blood through a bed composed of microcrystalline cellulose and alpha-cellulose. The method is rapid, reliable, removes over 99.75 per cent of the leukocytes from blood, and does not seem selectively to retain reticulocytes or to release a significant proportion of leukocyte enzymes. Most of the platelets are also removed from anticoagulated blood, and platelet-free red cells can be obtained by passing defibrinated blood over the microcrystalline cellulose-alpha-cellulose bed.