Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
Suppression of nbe-miR1919c-5p Expression in Nicotiana benthamiana Enhances Tobacco Curly Shoot Virus and Its Betasatellite Co-Infection
复制标题
抑制本塞姆氏烟草中的 nbe-miR1919c-5p 表达可增强烟草卷芽病毒及其 β 卫星联合感染
DOI：
10.3390/v12040392
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发表时间：
2020-04-01
期刊：
影响因子：
4.7
通讯作者：
Qing, Ling
中科院分区：
文献类型：
--
作者：
Du, Jiang;Wu, Rui;Qing, Ling
MicroRNAs （miRNAs）是长度为21-25个核苷酸的非编码但具有功能的RNA分子。mirna在多种植物生物学过程中发挥着重要的调控作用。为了揭示nbe-miR1919c-5p与烟草卷枝病毒（TbCSV）及其betasat卫星（TbCSB） dna积累以及病毒症状发展之间的关系，我们利用pvx和trv短串联靶模拟（STTM）技术研究了nbe-miR1919c-5p在烟草卷枝病毒和TbCSB共侵染过程中的功能。利用这两种技术抑制nbe-miR1919c-5p的表达，可增强TbCSV和TbCSB共同侵染诱导的烟叶卷曲症状。此外，抑制nbe-miR1919c-5p的表达可增强侵染植株TbCSV和TbCSB DNA的积累。我们的研究结果有助于进一步了解nbe-miR1919c-5p在TbCSV和TbCSB合并感染中的功能。
MicroRNAs (miRNAs) are non-coding but functional RNA molecules of 21-25 nucleotides in length. MiRNAs play significant regulatory roles in diverse plant biological processes. In order to decipher the relationship between nbe-miR1919c-5p and the accumulations of tobacco curly shoot virus (TbCSV) and its betasatellite (TbCSB) DNAs, as well as viral symptom development, we investigated the function of nbe-miR1919c-5p during TbCSV and TbCSB co-infection in plants using a PVX-and a TRV-based short tandem target mimic (STTM) technology. Suppression of nbe-miR1919c-5p expression using these two technologies enhanced TbCSV and TbCSB co-infection-induced leaf curling symptoms in Nicotiana benthamiana plants. Furthermore, suppression of nbe-miR1919c-5p expression enhanced TbCSV and TbCSB DNA accumulations in the infected plants. Our results can advance our knowledge on the nbe-miR1919c-5p function during TbCSV and TbCSB co-infection.