NEUROGRANIN - IMMUNOCYTOCHEMICAL LOCALIZATION OF A BRAIN-SPECIFIC PROTEIN-KINASE-C SUBSTRATE
NEUROGRANIN - IMMUNOCYTOCHEMICAL LOCALIZATION OF A BRAIN-SPECIFIC PROTEIN-KINASE-C SUBSTRATE
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DOI：
10.1523/jneurosci.10-12-03782.1990
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发表时间：
1990-12-01
影响因子：
5.3
通讯作者：
BAUDIER, J
中科院分区：
文献类型：
--
作者：
REPRESA, A;DELOULME, JC;BAUDIER, J
研究了神经粒蛋白（以前称为 p 17）（一种脑特异性蛋白激酶 C (PKC) 底物）的发育表达和细胞定位。 使用神经颗粒蛋白多克隆抗体，通过对大鼠脑和神经元细胞培养物提取物进行免疫印迹来研究神经颗粒蛋白的发育表达。研究发现，神经粒蛋白的合成受发育调节，在胚胎和新生儿期不表达，在 2 至 3 周龄期间突然增加。 通过免疫组织化学，神经颗粒素主要存在于成年大鼠端脑中，特别位于大脑皮层、海马、纹状体和其他一些离散区域的神经元的细胞体和树突状突起中。丘脑、小脑和脑干中几乎不存在神经粒蛋白免疫反应性。 神经颗粒蛋白在神经元中的晚期发育表达和树突状定位是 I 型 PKC 同工酶的两个特征。 该蛋白在大鼠大脑整合区域的特定定位表明神经粒蛋白在中枢神经系统中具有高度专门化的功能。 提出了神经颗粒素在突触后水平 PKC 激活信号转导中的可能作用。
The developmental expression and the cellular localization of neurogranin (formerly designated p 17), a brain-specific protein kinase C (PKC) substrate, were investigated. The developmental expression of neurogranin was studied by immunoblotting of rat brain and neuronal cell-culture extracts using neurogranin polyclonal antibodies. Neurogranin synthesis was found to be developmentally regulated, with no expression in the embryonic and neonatal period and an abrupt increase between 2 and 3 weeks of age. By immunohistochemistry, neurogranin was found essentially in the adult rat telencephalon, specifically located in the cell bodies and dendritic processes of neurons of the cerebral cortex, hippocampus, striatum, and a few other discreet areas. Neurogranin immunoreactivity was nearly absent in the thalamus, cerebellum, and brain stem. The late developmental expression and the dendritic localization of neurogranin in neurons are 2 features that also characterize the type I PKC isozyme. The specific localization of the protein in integrative areas of the rat brain suggests a highly specialized function of neurogranin in the CNS. A possible role for neurogranin in the transduction of the PKC activation signals at the postsynaptic level is suggested.