Long-term RNA persistence in postmortem contexts.
Long-term RNA persistence in postmortem contexts.
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DOI：
10.1186/2041-2223-4-7
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发表时间：
2013-04-23
期刊：
影响因子：
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通讯作者：
Gilbert MT
中科院分区：
文献类型：
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作者：
Fordyce SL;Kampmann ML;van Doorn NL;Gilbert MT
核糖核酸（RNA）通常被认为是容易降解的脆弱分子。然而，有越来越多的文件长期（从几天到几个世纪）RNA持久性在各种环境和组织类型，因此，一些学科开始利用降解RNA。虽然其存活的原因还不完全清楚，但有几种可能的机制可以保护这种分子免受降解。然而，在研究了关于RNA死后不稳定性和衰变机制的文献后，很明显，有限的实验研究和评论提供了这些机制的概述。因此，在这篇综述中，我们概述了RNA生存长期死后的分子原因，并提供了具体的例子，RNA生存在法医，档案和考古背景。更好地了解RNA衰变的机制对于发展其长期生存的期望至关重要。
Ribonucleic acids (RNA) are generally considered fragile molecules that are readily degraded. However, there is growing documentation of long-term (from days to centuries) RNA persistence in a variety of contexts and tissue types, and as such a number of academic disciplines are beginning to exploit degraded RNA. While the reasons for its survival are not fully understood, there are several plausible mechanisms that would safeguard this molecule against degradation. However, after examining the literature available on the postmortem instability and decay mechanisms of RNA, it has become clear that limited experimental studies and no reviews offer an overview of these mechanisms. Hence in this review we outline molecular reasons for RNA surviving long-term postmortem, and provide specific examples of RNA survival in forensic, archival and archaeological contexts. A better understanding of the mechanisms of RNA decay will be crucial for developing expectations on its long-term survival.