TECHNIQUE FOR ESTIMATING TOTAL SPINE NUMBERS ON GOLGI-IMPREGNATED DENDRITES
TECHNIQUE FOR ESTIMATING TOTAL SPINE NUMBERS ON GOLGI-IMPREGNATED DENDRITES
复制标题
DOI：
10.1002/cne.901880403
复制
发表时间：
1979-01-01
影响因子：
2.5
通讯作者：
PETERS, A
中科院分区：
文献类型：
--
作者：
FELDMAN, ML;PETERS, A
树突棘的功能意义和它们对广泛的实验操作和病理状态的形态敏感性导致了一些研究，其中树突棘数量的计数已经进行。这些研究，在大多数情况下，只涉及那些刺从高尔基浸渍树突的不透明轴突出到树突两侧的清晰区域的计数。这种计数仅限于可见的棘，不能代表树突上棘的真实总数。代表性不足的程度与树突轴直径呈正相关，与棘长度呈负相关。这严重限制了树突之间，甚至同一树突的片段之间的棘密度的比较的有用性。一个几何为基础的方法，在大鼠树突棘总数可以估计合理的准确性，考虑到因素，如树突直径和脊柱长度。该技术需要以下主要步骤：对于给定长度的树突，在其上要计数棘，确定其中棘可见并且可以计数的侧翼区的体积;确定围绕树突轴并且包含所有棘（可见和不可见）的整个区的体积;以及从可见脊的数量进行比例外推以获得真实的总脊数量的估计。使用已知总棘数的树突对该技术的预测准确性进行的测试表明，可以实现偏离真实总棘数< 10%的估计。
The functional significance of dendritic spines and their morphological sensitivity to a wide spectrum of experimental manipulations and pathological states have led to a number of studies in which counts of dendritic spine number have been carried out. These studies have, for the most part, involved the enumeration of only those spines which protrude from the opaque shafts of Golgi-impregnated dendrites into the clear zones flanking the dendrite. Such counts, limited to only those spines which are visible, underrepresent the true total number of spines borne by the dendrites. The magnitude of underrepresentation correlates positively with dendritic shaft diameter and negatively with spine length. This seriously restricts the usefulness of comparisons of spine density between dendrites, or even between segments of the same dendrite. A geometrically based method is presented in rats whereby total dendritic spine numbers can be estimated with reasonable accuracy, taking into account factors such as dendrite diameter and spine length. The technique entails the following principal steps: a determination, for a given length of dendrite over which spines are to be enumerated, of the volume of the flanking zones in which spines are visible and can be counted; a determination of the volume of the entire zone which encircles the dendritic shaft and which contains all spines, both visible and not visible; and a proportional extrapolation from the number of visible spines to obtain an estimate of the true total spine number. Tests of the predictive accuracy of the technique using dendrites of known total spine number suggest that estimates which deviate from true total spine numbers by < 10% can be achieved.