Effects of Lead and Cadmium Combined Heavy Metals on Liver Function and Lipid Metabolism in Mice
Effects of Lead and Cadmium Combined Heavy Metals on Liver Function and Lipid Metabolism in Mice
复制标题
铅镉复合重金属对小鼠肝功能和脂质代谢的影响
DOI：
10.1007/s12011-022-03390-5
复制
发表时间：
2022-08-22
影响因子：
3.9
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
--
作者：
Chen, Huaguo;Zhu, Chengxiang;Zhou, Xin
尽管对铅 (Pb) 和镉 (Cd) 单独暴露进行了大量研究，但有关 Pb 和 Cd 联合暴露毒性的信息相对有限。本研究旨在探讨Pb-Cd复合暴露的毒性及其相应机制。采用Pb-Cd(50:1)皮下灌胃35 d建立小鼠重金属暴露模型。采用体重、饮食、毛发状态、精神状态、肝脏指数、血液学指标、生化指标及病理切片分析等综合评价毒性。然后，利用经典的氧化应激指标和脂质组学技术探讨其潜在机制。结果表明，Pb-Cd导致小鼠食欲不振、精神差、活动量明显减少、体重增加缓慢、毛发发炎或干燥。 Pb-Cd还会引起肝脏肿大，使天门冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）酶活性显着升高，引起肝脏病理改变。长期接触铅镉会导致血小板血细胞比容（PCT）、白细胞（WBC）、血小板（PLT）和单核细胞（MON）计数显着增加，红细胞（RBC）、血红蛋白（HGB）、血细胞比容（HCT）和淋巴细胞（LYM）计数减少。 Pb-Cd通过增加超氧化物歧化酶（SOD）和乳酸脱氢酶（LDH）的活性以及丙二醛（MDA）的含量来增加氧化应激。最后，Pb-Cd通过调节亚油酸、鞘脂和甘油脂代谢引发脂质代谢紊乱。
Although a large number of studies have been conducted on lead (Pb) and cadmium (Cd) exposure individually, information regarding the toxicity of combined Pb and Cd exposure is relatively limited. The present study aims to investigate the toxicity of Pb-Cd combination exposure and the corresponding mechanism. A heavy metal exposure model was established in mice by subcutaneous intragastric administration of Pb-Cd (50:1) for 35 days. Body weight, diet, hair state, mental state, liver index, haematological index, biochemical indicators and pathological section analysis were used to comprehensively evaluate toxicity. Then, classical oxidative stress indexes and lipidomics techniques were used to explore the potential mechanism. The results showed that Pb-Cd caused the mice to have low appetite, poor spirit, significantly reduced activity, slow weight gain and irritated or drying hair. Pb-Cd also caused liver enlargement, significantly increased aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities, and resulted in pathological changes to the liver. Prolonged Pb-Cd exposure led to significantly increased thrombocyte haematocrit (PCT), white blood cell (WBC), platelet (PLT) and monocyte (MON) counts and decreased red blood cell (RBC), haemoglobin (HGB), haematocrit (HCT) and lymphocyte (LYM) counts. Pb-Cd increased oxidative stress by increasing the activity of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) and the content of malondialdehyde (MDA). Finally, Pb-Cd triggered lipid metabolism disorders by regulating linoleic acid, sphingolipid and glycerolipid metabolism.