Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen
Diagnosis of American visceral leishmaniasis in humans and dogs using the recombinant Leishmania donovani A2 antigen
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DOI：
10.1016/s0732-8893(02)00410-8
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发表时间：
2002-08-01
影响因子：
2.9
通讯作者：
Fernandes, AP
中科院分区：
文献类型：
--
作者：
Carvalho, FAA;Charest, H;Fernandes, AP
A2蛋白在杜氏利什曼原虫的无鞭毛体阶段表达，主要由保守的重复元件组成。在这里，我们调查了一组美国内脏利什曼病(VL)血清中抗A2抗体的存在。用含有6个组氨酸残基(A2-His)标签的重组A2蛋白，对77%的症状性VL患者血清和87%利什曼原虫免疫荧光抗体试验(IFAT)阳性或寄生虫学评价阳性的犬血清进行了抗A2抗体的检测。15只有症状犬中有14只检出抗A2抗体，13只无症状犬中有10只检出抗A2抗体。此外，在无症状/抗A2阳性的动物中，9只动物的寄生虫也呈阳性。与其他常见犬病动物血清无明显交叉反应。我们的研究结果表明，A2蛋白是诊断新大陆VL的一种潜在工具，对狗的诊断将特别有用。(C)2002 Elsevier Science Inc.保留所有权利。
A2 proteins are expressed in the amastigote stage of Leishmania donovani and are composed predominantely by a conserved repetitive element. Here, we have investigated the presence of anti-A2 antibodies in a panel of American Visceral Leishmaniasis (VL) sera. Anti-A2 antibodies were detected by ELISA, using a recombinant A2 protein containing a tag of six histidine residues (A2-HIS), in 77% of patients sera with symptomatic VL and in 87% of sera from dogs that tested positive in Leishmania immunofluorescent-antibody test (IFAT) or in the parasitological evaluation. Anti-A2 antibodies were also detected in 14 out of 15 symptomatic and in 10 out of 13 asymptomatic dogs. In addition, among the asymptomatic/anti-A2 positive animals, 9 were also positive for the presence of parasites. No significant cross reactivity was observed with sera of animals with other common canine diseases. Our findings suggest that A2 protein is a potential tool for the diagnosis of VL in the New World, and will be particularly useful for diagnosis of dogs. (C) 2002 Elsevier Science Inc. All rights reserved.