Detergent solubilization of phosphatidylcholine bilayers in the fluid state:: Influence of the acyl chain structure
Detergent solubilization of phosphatidylcholine bilayers in the fluid state:: Influence of the acyl chain structure
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DOI：
10.1016/j.bbamem.2006.01.016
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发表时间：
2006-02-01
影响因子：
3.4
通讯作者：
Goni, Felx M.
中科院分区：
文献类型：
--
作者：
Ahyayauch, Hasna;Larijani, Banafshe;Goni, Felx M.
十七种不同的，化学定义的磷脂酰胆碱，分散在水介质中的大单层囊泡的形式，已被测试的非离子洗涤剂Triton X-100的溶解。温度（20摄氏度或45摄氏度）使得双层总是处于液体无序状态。对于每种情况，在平衡条件下测定增溶参数D-on（产生增溶开始的总去污剂：脂质摩尔比）和D-50（产生50%增溶的总去污剂：脂质摩尔比）。这两个参数一般平行变化。当双键被引入酰基链时，其他因素保持不变，溶解变得更加困难，即，需要更多的洗涤剂。顺式不饱和磷脂比相应的反式异构体需要更多的洗涤剂。增加C12和C16之间的饱和磷脂的链长适度降低去污剂/脂质比，导致溶解。酰基和烷基磷脂对Triton X-100增溶同样敏感。脂质链的顺序，通过DPH荧光偏振测量，似乎有利于增溶，也许是因为更有序的双层有一个较小的能力，以适应洗涤剂单体，而不分解成脂质洗涤剂混合胶束。(c)2006 Elsevier B. V.保留所有权利。
Seventeen different, chemically defined phosphatidylcholines, dispersed in aqueous medium in the form of large unilamellar vesicles, have been tested for solubilization by the non-ionic detergent Triton X-100. The temperatures (either 20 degrees C or 45 degrees C) were such that the bilayers were always in the liquid-disordered state. For each case, the solubilization parameters, D-on (total detergent: lipid mole ratio producing the onset of solubilization) and D-50 (total detergent: lipid mole ratio producing 50% solubilization), were determined under equilibrium conditions. Both parameters varied generally in parallel. When double bonds were introduced to the acyl chains, other factors remaining constant, solubilization became more difficult, i.e., more detergent was required. Cis-unsaturated phospholipids required more detergent than the corresponding transisomers. Increasing chain length in saturated phospholipids between C12 and C16 decreased moderately the detergent/lipid ratios causing solubilization. Acyl and alkyl phospholipids were equally susceptible to Triton X-100 solubilization. Lipid chain order, as measured by DPH fluorescence polarization, seemed to facilitate solubilization, perhaps because more ordered bilayers have a smaller capacity to accommodate detergent monomers without breaking down into lipid-detergent mixed micelles. (c) 2006 Elsevier B.V. All rights reserved.