Dbx1 Pre-Botzinger Complex Interneurons Comprise the Core Inspiratory Oscillator for Breathing in Unanesthetized Adult Mice
Dbx1 Pre-Botzinger Complex Interneurons Comprise the Core Inspiratory Oscillator for Breathing in Unanesthetized Adult Mice
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DOI：
10.1523/eneuro.0130-18.2018
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发表时间：
2018-05-01
期刊：
影响因子：
3.4
通讯作者：
Del Negro, Christopher A.
中科院分区：
文献类型：
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作者：
Vann, Nikolas C.;Pham, Francis D.;Del Negro, Christopher A.
脑干Pre-Botzinger复合体(PreBotC)产生吸气呼吸节律，但它的节律核心是哪些神经元？DBX1来源的神经元可能在节律的产生中扮演着重要的角色，这一观点在围产期发育阶段得到了很好的支持，但在成年后并未得到完全评估。我们在正常成年小鼠的Dbx1前BotC神经元中表达了Archaerho品素或通道视紫红质，以询问它们的功能。长时间的光抑制会减缓或停止呼吸，而长时间的光刺激会加速呼吸。短暂的吸气相光抑制比预期更早地唤起下一次呼吸，而短暂的呼气相光抑制延迟了下一次呼吸。相反，短暂的吸气相光刺激延长了吸气持续时间，推迟了下一次呼吸，而短暂的呼气相光刺激比预期的更早唤起下一次呼吸。由于它们控制着清醒的成年小鼠的呼吸频率和精确的呼吸时间，感觉运动反馈完好无损，Dbx1前BotC神经元构成了吸气振荡器的基本核心组件，这是与人类健康和生理直接相关的知识。
The brainstem pre-Botzinger complex (preBotC) generates inspiratory breathing rhythms, but which neurons comprise its rhythmogenic core? Dbx1-derived neurons may play the preeminent role in rhythm generation, an idea well founded at perinatal stages of development but incompletely evaluated in adulthood. We expressed archaerhodopsin or channelrhodopsin in Dbx1 preBotC neurons in intact adult mice to interrogate their function. Prolonged photoinhibition slowed down or stopped breathing, whereas prolonged photostimulation sped up breathing. Brief inspiratory-phase photoinhibition evoked the next breath earlier than expected, whereas brief expiratory-phase photoinhibition delayed the subsequent breath. Conversely, brief inspiratory-phase photostimulation increased inspiratory duration and delayed the subsequent breath, whereas brief expiratory-phase photostimulation evoked the next breath earlier than expected. Because they govern the frequency and precise timing of breaths in awake adult mice with sensorimotor feedback intact, Dbx1 preBotC neurons constitute an essential core component of the inspiratory oscillator, knowledge directly relevant to human health and physiology.