Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.
Transcriptional regulation of a promoter in the men gene cluster of Bacillus subtilis.
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枯草芽孢杆菌 men 基因簇启动子的转录调控。
DOI：
10.1128/jb.170.6.2742-2748.1988
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发表时间：
1988
影响因子：
3.2
通讯作者：
Taber,H
中科院分区：
文献类型：
--
作者：
Miller,P;Mueller,J;Hill,K;Taber,H
在转录水平上研究了枯草芽孢杆菌生长和产孢过程中men基因表达的调控。采用了两种不同的方法。(i)直接测量男性特异性mRNA的稳态水平。(ii)构建了men '-lacZ基因融合体。在这两种情况下，观察到men启动子活性在孢子形成开始时最大，此后不久下降。这些动力学与先前观察到的甲萘醌积累模式相似。从男性启动子的表达是独立的spo 0A和spo 0 H基因的产物的存在下，并通过添加葡萄糖和谷氨酰胺的培养基中增强。启动子区的DNA序列分析揭示了一个潜在的识别位点的主要营养形式的RNA聚合酶，但不是任何已知的次要聚合酶的形式。通过转录起始位点的高分辨率S1核酸酶作图证实了启动子序列在体内的功能。一个额外的序列元件被确定为共享的sdhA，citG，和ctaA启动子，并可能表明这些基因的表达中的共同调控机制。
The control of men gene expression during growth and sporulation of Bacillus subtilis was examined at the transcriptional level. Two different approaches were used. (i) Steady-state levels of men-specific mRNA were measured directly. (ii) A men'-lacZ gene fusion was constructed. In both cases, it was observed that men promoter activity was maximal at the onset of sporulation and declined soon thereafter. These kinetics were similar to the pattern of menaquinone accumulation previously observed. Expression from the men promoter was independent of the presence of the products of the spo0A and spo0H genes and was enhanced by addition of glucose and glutamine to the culture medium. DNA sequence analysis of the promoter region revealed a potential recognition site for the principal vegetative form of RNA polymerase but not for any of the known minor polymerase forms. The functionality in vivo of the promoter sequence was confirmed by high-resolution S1 nuclease mapping of the transcript start site. An additional sequence element was identified that is shared by the sdhA, citG, and ctaA promoters and may indicate a common regulatory mechanism in the expression of these genes.