Effects of secretome obtained from normoxia-preconditioned human mesenchymal stem cells in traumatic brain injury rats
Effects of secretome obtained from normoxia-preconditioned human mesenchymal stem cells in traumatic brain injury rats
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DOI：
10.1097/ta.0b013e318265d128
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Kuo, Jinn-Rung
中科院分区：
文献类型：
--
作者：
Chuang, Tai-Jen;Lin, Kao-Chang;Kuo, Jinn-Rung
背景技术背景：本研究的目的是研究从常氧预处理的人间充质干细胞中获得的分泌组是否会减轻液体冲击损伤诱导的创伤性脑损伤（TBI）。方法：麻醉的雄性Sprague-Dawley大鼠，在液体冲击脑损伤（TBI）发生后立即分为两大组，并静脉内给予溶剂溶液（1 mL）或分泌组（500 μ g）。另一组大鼠用作假手术对照。通过斜面测试评价功能结果，如运动（最大抓握角）。氯化三苯基四氮唑染色计算细胞梗死体积。通过免疫荧光染色检测皮质神经元丢失、凋亡和神经营养因子如血管上皮生长因子（VEGF）的表达。结果：与假手术对照组相比，分泌体治疗可明显减轻TBI后大鼠的运动障碍和脑梗死。分泌组治疗也显著减少TBI诱导的神经元丢失和凋亡。此外，VEGF阳性细胞在缺血皮层TBI后进一步显着增加分泌体therapeutic.CONCLUSION：我们的研究结果表明，静脉注射分泌体从常氧预处理的人骨髓间充质干细胞可以改善TBI诱导的大鼠通过减少神经元细胞丢失和凋亡，促进VEGF的产生，导致功能的结果改善。我们还建议，常氧预处理的人间充质干细胞分泌组可能是创伤性脑损伤的一个有前途的治疗策略。（J Trauma Acute Care Surg.2012; 73：1161-1167.版权所有（c）2012 Lippincott威廉姆斯& Wilkins）
BACKGROUND: The aim of the present study was to investigate whether secretome obtained from normoxia-preconditioned human mesenchymal stem cells causes attenuation of traumatic brain injury (TBI), induced by fluid percussion injury.METHODS: Anaesthetized male Sprague-Dawley rats, immediately after the onset of fluid percussion TBI, were divided into two major groups and given the vehicle solution (1 mL) or secretome (500 mu g) intravenously. Another group of rats was used as sham-operated controls. The functional outcome such as motor (maximum grasp angle) was evaluated by an inclined plane test. Cellular infarction volume was calculated by triphenyltetrazolium chloride staining. Neuronal loss, apoptosis, and neurotrophic factor such as vascular epithelial growth factor (VEGF) expression in the cortex were measured by immunofluorescence staining. All the parameters were assessed on Day 3 after injury.RESULTS: Compared with those of the sham-operated controls, the motor deficits and cerebral infarction of rats after TBI were significantly attenuated by secretome therapy. The TBI-induced neuronal loss and apoptosis were also significantly reduced by secretome therapy. Furthermore, VEGF-positive cells in the ischemic cortex after TBI were further significantly increased by secretome therapy.CONCLUSION: Our results demonstrate that intravenous injection of secretome from normoxia-preconditioned human mesenchymal stem cells may ameliorate TBI-induced rats by reducing neuronal cell loss and apoptosis and promoting VEGF production, resulting in functional outcome improvement. We also recommend that secretome from normoxia-preconditioned human mesenchymal stem cells could be a promising treatment strategy for traumatic brain injury. (J Trauma Acute Care Surg. 2012; 73: 1161-1167. Copyright (c) 2012 by Lippincott Williams & Wilkins)