Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression
Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression
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STN7/STN8 激酶靶标的鉴定揭示了电子传递、代谢和基因表达之间的联系
DOI：
10.1111/tpj.13536
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
S. Baginsky
中科院分区：
文献类型：
--
作者：
A. Schönberg;Anja Rödiger;Wiebke Mehwald;Johann Galonska;G. Christ;Stefan Helm;Domenika Thieme;Petra Majovsky;W. Hoehenwarter;S. Baginsky
类囊体相关激酶STN 7和STN 8参与光合电子传递对变化的光条件的短期和长期适应。在这里，我们报告的STN 7/STN 8在体内的目标，连接光合电子传递代谢和基因表达的鉴定。比较磷酸化蛋白质组学与stn 7和stn 8单，双突变体确定了两种蛋白酶，一种RNA结合蛋白，核糖体蛋白，大亚基的Rubisco和铁氧还蛋白-NADP还原酶作为靶点的类囊体相关激酶。在stn 7单突变体中，上述三种蛋白质的磷酸化可以被STN 8部分补充，尽管效率较低，而其余三种蛋白质的磷酸化严格依赖于STN 7。STN 7依赖性磷酸化位点的性质类似于在-1位需要甘氨酸或另一种小的疏水氨基酸的磷酸化捕光复合物蛋白的性质。我们的分析揭示了STN 7/STN 8激酶作为光合电子传递，其直接下游汇和影响代谢物积累和基因表达的长期适应过程之间的介质。
The thylakoid-associated kinases STN7 and STN8 are involved in short- and long-term acclimation of photosynthetic electron transport to changing light conditions. Here we report the identification of STN7/STN8 in vivo targets that connect photosynthetic electron transport with metabolism and gene expression. Comparative phosphoproteomics with the stn7 and stn8 single and double mutants identified two proteases, one RNA-binding protein, a ribosomal protein, the large subunit of Rubisco and a ferredoxin-NADP reductase as targets for the thylakoid-associated kinases. Phosphorylation of three of the above proteins can be partially complemented by STN8 in the stn7 single mutant, albeit at lower efficiency, while phosphorylation of the remaining three proteins strictly depends on STN7. The properties of the STN7-dependent phosphorylation site are similar to those of phosphorylated light-harvesting complex proteins entailing glycine or another small hydrophobic amino acid in the -1 position. Our analysis uncovers the STN7/STN8 kinases as mediators between photosynthetic electron transport, its immediate downstream sinks and long-term adaptation processes affecting metabolite accumulation and gene expression.
DOI：
10.1074/mcp.m114.040352
发表时间：
2015-03
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Roitinger E;Hofer M;Köcher T;Pichler P;Novatchkova M;Yang J;Schlögelhofer P;Mechtler K
通讯作者：
Mechtler K
影响因子：
3.7
作者：
Schönberg A;Bergner E;Helm S;Agne B;Dünschede B;Schünemann D;Schutkowski M;Baginsky S
通讯作者：
Baginsky S