The melanocortin receptor-1 gene but not the proopiomelanocortin gene is expressed in melanoblasts and contributes their differentiation in the mouse skin
The melanocortin receptor-1 gene but not the proopiomelanocortin gene is expressed in melanoblasts and contributes their differentiation in the mouse skin
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DOI：
10.1111/j.1600-0749.2004.00179.x
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发表时间：
2004-12-01
期刊：
影响因子：
--
通讯作者：
Hotta, E
中科院分区：
文献类型：
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作者：
Hirobe, T;Takeuchi, S;Hotta, E
将α-促黑素细胞激素 (α-MSH) 添加到源自 0.5 日龄 C57BL/10JHir 小鼠表皮细胞悬浮液的黑素细胞的无血清原代培养物中，诱导其分化。使用逆转录聚合酶链式反应进行的分析表明，在添加α-MSH之前，黑素细胞特异性α-MSH受体基因黑皮质素受体-1（MC1-R）已经开始表达，此外，在添加α-MSH后没有观察到MC1-R基因的上调。然而，在添加 α-MSH 之前或之后没有观察到阿片黑皮质素原 (POMC) 基因的表达。调查13日龄胚胎至5.5日龄新生儿背侧皮肤表皮和真皮中MC1-R和POMC基因的表达情况。 MC1-R基因的表达首先在13天胚胎的表皮中观察到，并在出生后0.5天逐渐增加，此后保持恒定。相比之下，真皮中MC1-R基因的表达首先在16天大的胚胎中观察到，并在出生后逐渐增加至3.5天，此后保持恒定。然而，在任何年龄的测试小鼠的背部皮肤的表皮或真皮中均未观察到POW基因的表达。这些结果表明MC1-R基因的表达，而不是POW基因的表达，在小鼠皮肤黑素细胞分化的调节中发挥重要作用。
Alpha-melanocyte stimulating hormone (alpha-MSH) added to serum-free primary culture of melanoblasts derived from epidermal cell suspensions of 0.5 d old C57BL/10JHir mice induced their differentiation. Analysis using the reverse transcription-polymerase chain reaction showed that the expression of the melanocyte-specific alpha-MSH receptor gene, melanocortin receptor-1 (MC1-R), had already been initiated before addition of alpha-MSH, and, in addition, no up-regulation of the MC1-R gene was observed after addition of a-MSH. However, no expression of the proopiomelanocortin (POMC) gene was observed before or after the addition of alpha-MSH. The expression of the MC1-R and POMC genes in the epidermis and dermis of the dorsal skin was surveyed from 13 d old embryos to 5.5 d old neonates. The expression of the MC1-R gene was first observed in the epidermis of 13 d old embryos, and gradually increased up to 0.5 d after birth, and thereafter remained constant. By contrast, the expression of the MC1-R gene in the dermis was first observed in 16 d old embryos, and gradually increased up to 3.5 d after birth, and thereafter remained constant. However, no expression of the POW gene was observed in the epidermis or dermis of the dorsal skin at any age of mice tested. These results suggest that the expression of the MC1-R gene, but not of the POW gene, plays an important role in the regulation of melanocyte differentiation in mouse skin.