A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin
A model of the acrosome reaction progression via the acrosomal membrane-anchored protein equatorin
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DOI：
10.1530/rep-09-0434
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发表时间：
2010-03-01
期刊：
影响因子：
3.8
通讯作者：
Toshimori, Kiyotaka
中科院分区：
文献类型：
--
作者：
Yoshida, Keiichi;Ito, Chizuru;Toshimori, Kiyotaka
建立一个可靠的、渐进的顶体反应模型是非常重要的。在这里，我们提出了一个进展模型的顶体反应围绕顶体膜锚定蛋白赤道蛋白（MN 9），比较染色模式跟踪MN 9抗体免疫荧光跟踪花生凝集素（PNA）-FITC。在顶体反应之前，赤道蛋白存在于前顶体和赤道段。由于精子在透明带上表现出不同的染色模式，MN 9免疫染色模式分为四个阶段：初始，早期，先进，和最终。随着顶体反应从初始阶段到早期阶段的进展，赤道蛋白从前顶体的周边区域向赤道段的中心扩散，在晚期阶段逐渐遍及赤道段的整个区域，最后在最后阶段均匀地分布在赤道段。相反，PNA-FITC信号从顶体的周边区域向整个赤道段传播得更快，同时染色强度降低，最终在最后阶段变得较弱。MN 9-免疫金电子显微镜显示，在顶体反应的晚期，在被无定形物质包围的杂交囊泡上有赤道素。赤道蛋白的分子量从40-60 kDa降低到35 kDa，并且35 kDa赤道蛋白的信号强度随着顶体反应的进行而增加。因此，所建立的基于赤道素的进展模型将是有用的，不仅用于分析赤道素的行为，但也参与顶体反应的其他感兴趣的分子。生殖（2010年）139 533-544
It is important to establish a reliable and progressive model of the acrosome reaction. Here, we present a progression model of the acrosome reaction centering around the acrosomal membrane-anchored protein equatorin (MN9), comparing the staining pattern traced by MN9 antibody immunofluorescence with that traced by Arachis hypogaea agglutinin (PNA)-FITC. Prior to the acrosome reaction, equatorin was present in both the anterior acrosome and the equatorial segment. Since sperm on zona pellucida showed various staining patterns, MN9-immunostaining patterns were classified into four stages: initial, early, advanced, and final. As the acrosome reaction progressed from the initial to the early stage, equatorin spread from the peripheral region of the anterior acrosome toward the center of the equatorial segment, gradually over the entire region of the equatorial segment during the advanced stage, and finally uniformly at the equatorial segment at the final stage. In contrast, the PNA-FITC signals spread more quickly from the peripheral region of the acrosome toward the entire equatorial segment, while decreasing in staining intensity, and finally became weak at the final stage. MN9-immunogold electron microscopy showed equatorin on the hybrid vesicles surrounded by amorphous substances at advanced stage of acrosome reaction. Equatorin decreased in molecular mass from 40-60 to 35 kDa, and the signal intensity of 35 kDa equatorin increased as the acrosome reaction progressed. Thus, the established equatorin-based progression model will be useful for analyzing not only the behavior of equatorin but also of other molecules of interest involved in the acrosome reaction. Reproduction (2010) 139 533-544