Use of the API 20E system to identify veterinary Enterobacteriaceae
Use of the API 20E system to identify veterinary Enterobacteriaceae
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使用 API 20E 系统识别兽用肠杆菌科细菌
DOI：
10.1128/jcm.12.1.10-14.1980
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发表时间：
1980
影响因子：
9.4
通讯作者：
M. Collins
中科院分区：
文献类型：
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作者：
E. C. Swanson;M. Collins
在API 20 E试纸上对从国家兽医诊断实验室获得的共计503种兽医肠道细菌病原体进行了检测，以确定这种快速微量鉴定系统是否可用于兽医临床微生物学。API 20 E试剂条准确鉴定了96%的兽用分离株，错误鉴定了3%。API系统和诊断实验室对85%分离株的鉴定结果一致，16%分离株的鉴定结果不一致，1%未被API试剂条鉴定。鉴定上的差异主要发生在区分克雷伯氏菌属和肠杆菌属以及肠杆菌属和大肠埃希氏菌。这些不一致通常是由于诊断实验室而不是API系统的错误鉴定。比较了人和兽用分离株之间的生物型差异。在几个生化反应中观察到显著差异。观察到的主要差异为E.大肠杆菌菌株产鸟氨酸脱羧酶和蜜二糖发酵。沙门氏菌的最大差异发生在精氨酸二氢水解酶的生产，柠檬酸盐的利用，和肌醇发酵，而肺炎克雷伯氏菌的主要差异，注意到在尿素酶的生产和硝酸盐还原。然而，这些生物型差异并不影响API试剂条上微生物的准确鉴定。
A total of 503 veterinary enteric bacterial pathogens obtained from state veterinary diagnostic laboratories were tested on API 20E strips to determine whether this rapid microidentification system could be utilized for veterinary clinical microbiology. The API 20E strip accurately identified 96% of the veterinary isolates and misidentified 3%. Identifications by the API system and the diagnostic laboratories were in agreement in 85% of the isolates, disagreement on 16% of the isolates, and 1% were not identified by the API strip. Differences in identification occurred primarily in distinguishing between Klebsiella and Enterobacter and between Enterobacter and Escherichia coli. These disagreements were most often due to incorrect identifications by the diagnostic laboratory rather than by the API system. Biotype differences between human and veterinary isolates were compared. Significant differences were noted in several biochemical reactions. The main differences observed for E. coli isolates were in ornithine decarboxylase production and melibiose fermentation. The largest differences for Salmonella occurred in arginine dihydrolase production, citrate utilization, and inositol fermentation, whereas for Klebsiella pneumoniae the main differences were noted in urease production and nitrate reduction. These biotype differences, however, did not affect the accurate identification of organisms on the API strip.