Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin.
Flagellar motility requires the cAMP-dependent phosphorylation of a heat-stable NP-40-soluble 56 kd protein, axokinin.
复制标题
鞭毛运动需要热稳定的 NP-40 可溶性 56 kd 蛋白轴突蛋白的 cAMP 依赖性磷酸化。
DOI：
10.1016/0092-8674(84)90509-9
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发表时间：
1984
期刊：
影响因子：
64.5
通讯作者：
Means,AR
中科院分区：
文献类型：
--
作者：
Tash,JS;Kakar,SS;Means,AR
使用 NP-40 处理的狗精子作为模型，CAMP 对重新激活鞭毛运动的刺激作用已被证明取决于 56 kd 的热稳定 NPQO 可溶蛋白的 CAMP 依赖性磷酸化。通过二维聚丙烯酰胺凝胶电泳检查用 Y-~* PATP 磷酸化的 NP-40 提取蛋白，揭示了 56 kd 处的主要 CAMP 依赖性磷酸肽。这是所有组织的 NP-40 提取物中唯一共有的 CAMP 依赖性磷蛋白，显示出 CAMP 依赖性鞭毛运动刺激。这些细胞和组织包括海胆、狗和人类精子，以及狗的气管和视网膜。此外，骨骼肌、大脑和肝脏等组织的非刺激性提取物中不存在这种磷蛋白。我们得出的结论是，56 kd 肽的 CAMP 依赖性磷酸化不仅代表精子的主要调节成分，而且代表其他类型轴丝运动的主要调节成分。
Using NP-40-treated dog sperm as a model, the stimulatory effect of CAMP upon reactivated flagellar motility has been shown to be dependent upon the CAMP-dependent phosphorylation of a heat-stable NPQO-soluble protein of 56 kd. Examination by twodimensional polyacrylamide gel electrophoresis of NP-40 extract proteins phosphorylated with Y-~* PATP revealed a major CAMP-dependent phosphopeptide at 56 kd. This is the only CAMP-dependent phosphoprotein common to NP-40 extracts of all tissues that show CAMP-dependent stimulation of flagellar motility. These cells and tissues include sea urchin, dog, and human sperm, as well as dog trachea and retina. Moreover, this phosphoprotein is absent in nonstimulatory extracts from tissues such as skeletal muscle, brain, and liver. We conclude that the CAMP-dependent phosphorylation of the 56 kd peptide represents a major regulatory component of not only sperm but other types of axonemal motility as well.