Molecular Concentration Determination Using Long-Interval Chemical Exchange Inversion Transfer (CEIT) NMR Spectroscopy
Molecular Concentration Determination Using Long-Interval Chemical Exchange Inversion Transfer (CEIT) NMR Spectroscopy
复制标题
使用长间隔化学交换反转转移 (CEIT) 核磁共振波谱法测定分子浓度
DOI：
10.1021/acs.jpclett.1c02239
复制
发表时间：
2021-09-02
影响因子：
5.7
通讯作者：
Zhou, Xin
中科院分区：
文献类型：
--
作者：
Jiang, Weiping;Guo, Qianni;Zhou, Xin
功能化的超极化氙“笼”分子经常被用于生物分子和微环境特性的超灵敏检测。然而，快速准确地测量分子浓度仍然是一个挑战。在这里，我们报告了一种使用长间隔化学交换倒置转移（CEIT）核磁共振光谱的分子浓度测量方法。只需2次扫描即可定量测量分子浓度，与传统的Hyper-CEST（化学交换饱和转移）z谱方法相比，采集时间缩短了约10倍。此外，我们发现，当CEIT效应为1-1/e或接近该效应时，浓度测定的准确性最好，仅对循环次数进行一步优化，CrA-(COOH)(6)的相对偏差小于+/- 1%。该方法能够高效、准确地测定分子浓度，为快速定量分子成像应用提供了一种潜在的方法。
Functionalized hyperpolarized xenon "cage" molecules have often been used for ultrasensitive detection of biomolecules and microenvironment properties. However, the rapid and accurate measurement of molecule concentration is still a challenge. Here, we report a molecule concentration measurement method using long-interval chemical exchange inversion transfer (CEIT) NMR spectroscopy. The molecule concentration can be quantitatively measured with only 2 scans, which shortens the acquisition time by about 10 times compared to conventional Hyper-CEST (chemical exchange saturation transfer) z-spectrum method. Moreover, we found that the accuracy of concentration determination would be the best when the CEIT effect is 1-1/e or close to it, and a relative deviation of CrA-(COOH)(6) less than +/- 1% has been achieved by only a one-step optimization of the number of cycles. The proposed method enables efficient and accurate determination of molecule concentration, which provides a potential way for rapid quantitative molecular imaging applications.