Bright Mono-aqua Europium Complexes Based on Triazacyclononane That Bind Anions Reversibly and Permeate Cells Efficiently
Bright Mono-aqua Europium Complexes Based on Triazacyclononane That Bind Anions Reversibly and Permeate Cells Efficiently
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DOI：
10.1002/chem.201301273
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发表时间：
2013-07-15
影响因子：
4.3
通讯作者：
Walton, James W.
中科院分区：
文献类型：
--
作者：
Butler, Stephen J.;McMahon, Brian K.;Walton, James W.
在330-355nm范围内激发后，由七齿N5O2配体制备了一系列五种铕（III）配合物，在水中具有超过10mM(-1)cm（-1）的亮度。几种氧阴离子的结合已经通过发射光谱滴定分析进行了评估，与简单羧酸盐、乳酸盐和柠檬酸盐的结合涉及在配位水位移后的共同连接模式。对碳酸氢盐的选择性可以快速测定人血清中的这种阴离子，K-d=37mM （295K）。这些复合物被迅速内化到哺乳动物细胞中，并在早期时间点表现出线粒体定位，在几小时后迁移，显示出主要的溶酶体分布。在此，我们报道了强发射铕（III）配合物的合成和配位行为，该配合物在水介质中结合氧阴离子，其亲和力和选择性与先前研究的系统明显不同。
A series of five europium(III) complexes has been prepared from heptadentate N5O2 ligands that possess a brightness of more than 10mM(-1)cm(-1) in water, following excitation over the range =330-355nm. Binding of several oxy anions has been assessed by emission spectral titrimetric analysis, with the binding of simple carboxylates, lactate and citrate involving a common ligation mode following displacement of the coordinated water. Selectivity for bicarbonate allows the rapid determination of this anion in human serum, with K-d=37mM (295K). The complexes are internalised quickly into mammalian cells and exhibit a mitochondrial localisation at early time points, migrating after a few hours to reveal a predominant lysosomal distribution. Herein, we report the synthesis and complexation behaviour of strongly emissive europium (III) complexes that bind oxy-anions in aqueous media with an affinity and selectivity profile that is distinctively different from previously studied systems.