The effect of melatonin on lipid peroxidation during radiotherapy in female rats
The effect of melatonin on lipid peroxidation during radiotherapy in female rats
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DOI：
10.1007/bf02743581
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发表时间：
1999-06
影响因子：
3.1
通讯作者：
H. Kaya;N. Delibaş;M. Serteser;E. Ulukaya;O. Özkaya
中科院分区：
文献类型：
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作者：
H. Kaya;N. Delibaş;M. Serteser;E. Ulukaya;O. Özkaya
背景放射治疗是原发性或继发性卵巢功能衰竭的原因之一，因此在接受全身或盆腔放射治疗的患者中保护卵巢功能具有重要意义。在这项研究中，我们研究了褪黑激素在放疗引起的全身和卵巢氧化损伤中的作用。材料与方法雌性大鼠18只，随机分为3组，每组6只。第一组为对照组，不进行治疗，第二组仅进行2x360 cGy的全身放疗，第三组放疗加褪黑素。检测血液和卵巢组织中丙二醛（MDA）水平作为自由基（FR）损伤的指标。测定血中红细胞超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）水平作为抗氧化水平的指标。结果放疗引起大鼠血液和卵巢组织MDA水平显著升高（p< 0.001）。放疗加褪黑素组MDA水平降低（p< 0.05）。单纯放疗组SOD、GPX水平降低不显著，放疗加褪黑素组SOD、GPX水平升高显著（p< 0.01、p< 0.05）。结论褪黑素可降低大鼠放疗后升高的MDA水平，增加参与抗氧化系统的SOD和GPX水平。
BackgroundBecause radiotherapy is one of the causes of primary or secondary ovarian failure, protection of ovarian functions in the patients receiving total body or pelvic radiotherapy is of importance. In this study, we investigated the role of melatonin in the oxidative damage in both whole body and ovaries, which is caused by radiotherapy.Materials and MethodsEighteen female rats were divided into 3 groups, each of which consisted of 6 rats. First group was control group receiving no treatment, second group received total body radiotherapy (RT) by 2× 360 cGy only and third group received radiotherapy plus melatonin. Malondialdehyde (MDA) levels in both blood and ovarian tissue were detected as the indicator of free radical (FR) damage. Levels of erythrocyte superoxide dismutase (SOD) and glutathion peroxidase (GPX) in blood were measured as the indicators of antioxidant level.ResultsRadiotherapy caused a significant increase in the levels of MDA in blood and ovarian tissue (p< 0.001). However, MDA levels decreased in the radiotherapy plus melatonin group (p< 0.05). SOD and GPX levels decreased insignificantly in the radiotherapy only group while they increased in the radiotherapy plus melatonin group significantly (p< 0.01 and p< 0.05, respectively).ConclusionMelatonin, in rats, reduced the level of MDA, which is elevated by radiotherapy and increased the levels of SOD and GPX, which are involved in the antioxidant system.