Plasma Surface Chemical Treatment of Electrospun Poly(L-Lactide) Microfibrous Scaffolds for Enhanced Cell Adhesion, Growth, and Infiltration
Plasma Surface Chemical Treatment of Electrospun Poly(L-Lactide) Microfibrous Scaffolds for Enhanced Cell Adhesion, Growth, and Infiltration
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DOI：
10.1089/ten.tea.2011.0725
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发表时间：
2013-05-01
影响因子：
4.1
通讯作者：
Li, Song
中科院分区：
文献类型：
--
作者：
Cheng, Qian;Lee, Benjamin Li-Ping;Li, Song
用Ar或ArNH 3/H-2等离子体处理静电纺丝法制备的聚乳酸（PLLA）微纤维支架，以增强细胞的附着、生长和浸润。使用测角法、原子力显微镜（AFM）和X射线光电子能谱（XPS）测量来评估通过等离子体处理对支架表面化学的改性。AFM和XPS测量结果表明，这两种等离子体处理增加了亲水性，而不影响纤维结构的完整性和纤维粗糙度，而Ar-NH3/H-2等离子体处理还导致与胺基的表面功能化。牛主动脉内皮细胞和牛平滑肌细胞在等离子体处理的PLLA支架上的培养研究表明，Ar和Ar-NH3/H-2等离子体处理都促进了细胞在细胞附着的初始阶段的铺展，更重要的是，增加了细胞的生长速率，尤其是Ar等离子体处理。体外细胞浸润研究表明，这两种等离子体处理有效地增强了细胞迁移到微纤维支架。涉及在Sprague-Dawley大鼠皮肤下皮下植入等离子体处理的PLLA支架的体内实验也显示出增加的细胞浸润。本研究的结果表明，PLLA微纤维支架与温和的Ar或Ar-NH3/H-2等离子体的表面处理可能有重要的意义，在组织工程。生物活性因子的进一步修饰将改善支架的功能，以用于特定的应用。
Poly(l-lactide) (PLLA) microfibrous scaffolds produced by electrospinning were treated with mild Ar or ArNH3/H-2 plasmas to enhance cell attachment, growth, and infiltration. Goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS) measurements were used to evaluate the modification of the scaffold surface chemistry by plasma treatment. AFM and XPS measurements showed that both plasma treatments increased the hydrophilicity without affecting the integrity of the fibrous structure and the fiber roughness, whereas Ar-NH3/H-2 plasma treatment also resulted in surface functionalization with amine groups. Culture studies of bovine aorta endothelial cells and bovine smooth muscle cells on the plasma-treated PLLA scaffolds revealed that both Ar and Ar-NH3/H-2 plasma treatments promoted cell spreading during the initial stage of cell attachment and, more importantly, increased the cell growth rate, especially for Ar plasma treatment. In vitro cell infiltration studies showed that both plasma treatments effectively enhanced cell migration into the microfibrous scaffolds. In vivo experiments involving the subcutaneous implantation of plasma-treated PLLA scaffolds under the skin of Sprague-Dawley rats also showed increased cell infiltration. The results of this study indicate that surface treatment of PLLA microfibrous scaffolds with mild Ar or Ar-NH3/H-2 plasmas may have important implications in tissue engineering. Further modifications with bioactive factors should improve the functions of the scaffolds for specific applications.