Biogeography of Deep-sea benthic bacteria at regional scale (LTER HAUSGARTEN, Fram Strait, Arctic).
Biogeography of Deep-sea benthic bacteria at regional scale (LTER HAUSGARTEN, Fram Strait, Arctic).
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DOI：
10.1371/journal.pone.0072779
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Ramette A
中科院分区：
文献类型：
--
作者：
Jacob M;Soltwedel T;Boetius A;Ramette A
关于深海生物分布的空间尺度的知识仍然很少，但与了解扩散、生境范围和生态过程密切相关。我们研究了区域空间分布模式的底栖细菌群落和共变的环境参数，如水深，生物量和能源供应在北极长期生态研究（LTER）网站豪斯加滕（东弗拉姆海峡）。从水深（水深1，284 - 3，535米，长54公里）和纬度样带（水深12，500米，长123公里）的13个站点采集了样本。454大规模平行标签测序（MPTS）和自动核糖体基因间间隔区分析（ARISA）相结合，以描述丰富和罕见的类型塑造细菌群落。这种空间抽样方案允许检测高达99%的估计丰富的门和类水平。在操作分类单位的分辨率（97%的序列同一性; OTU3%）只有36%的Chao 1估计的丰富度被恢复，表明高多样性，主要是由于罕见的类型（62%的所有OTU3%）。因此，在任何两个采样站之间也观察到细菌群落的高周转率（平均替换79%的OTU 3%），但在该区域内观察到与空间距离没有直接相关性。随着水深的增加，细菌群落组成和结构差异显着沿着测深样带。疣微菌和浮游菌的相对序列丰度随水深的增加而显著降低，而去铁菌的相对序列丰度随水深的增加而显著增加。能量供应，估计从沉积物中的植物碎屑色素浓度，部分解释了群落结构的变化。总的来说，这项研究表明，在相对较小的空间尺度（数十公里）上，独特的细菌类型比例很高，并支持LTER站点HAUSGARTEN的采样设计，以研究世界海洋这一快速变化区域的细菌群落变化。
Knowledge on spatial scales of the distribution of deep-sea life is still sparse, but highly relevant to the understanding of dispersal, habitat ranges and ecological processes. We examined regional spatial distribution patterns of the benthic bacterial community and covarying environmental parameters such as water depth, biomass and energy availability at the Arctic Long-Term Ecological Research (LTER) site HAUSGARTEN (Eastern Fram Strait). Samples from 13 stations were retrieved from a bathymetric (1,284–3,535 m water depth, 54 km in length) and a latitudinal transect (∼ 2,500 m water depth; 123 km in length). 454 massively parallel tag sequencing (MPTS) and automated ribosomal intergenic spacer analysis (ARISA) were combined to describe both abundant and rare types shaping the bacterial community. This spatial sampling scheme allowed detection of up to 99% of the estimated richness on phylum and class levels. At the resolution of operational taxonomic units (97% sequence identity; OTU3%) only 36% of the Chao1 estimated richness was recovered, indicating a high diversity, mostly due to rare types (62% of all OTU3%). Accordingly, a high turnover of the bacterial community was also observed between any two sampling stations (average replacement of 79% of OTU3%), yet no direct correlation with spatial distance was observed within the region. Bacterial community composition and structure differed significantly with increasing water depth along the bathymetric transect. The relative sequence abundance of Verrucomicrobia and Planctomycetes decreased significantly with water depth, and that of Deferribacteres increased. Energy availability, estimated from phytodetrital pigment concentrations in the sediments, partly explained the variation in community structure. Overall, this study indicates a high proportion of unique bacterial types on relatively small spatial scales (tens of kilometers), and supports the sampling design of the LTER site HAUSGARTEN to study bacterial community shifts in this rapidly changing area of the world’s oceans.
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影响因子：
4.8
作者：
BORCARD, D;LEGENDRE, P;DRAPEAU, P
通讯作者：
DRAPEAU, P
DOI：
10.1016/j.dsr.2006.09.007
发表时间：
2007-01-01
影响因子：
2.4
作者：
Hoste, Eveline;Vanhove, Sandra;Vanreusel, Ann
通讯作者：
Vanreusel, Ann
影响因子：
3.6
作者：
AMBROSE, WG;RENAUD, PE
通讯作者：
RENAUD, PE
影响因子：
2.5
作者：
Ingels, Jeroen;Van den Driessche, Pieter;Vanreusel, Ann
通讯作者：
Vanreusel, Ann
影响因子：
64.8
作者：
BILLETT, DSM;LAMPITT, RS;MANTOURA, RFC
通讯作者：
MANTOURA, RFC