Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum
Immunolocalization of the fatty acid-binding protein Sj-FABPc within adult Schistosoma japonicum
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DOI：
10.1017/s0031182097008925
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发表时间：
1997-07-01
期刊：
影响因子：
2.4
通讯作者：
McManus, DP
中科院分区：
文献类型：
--
作者：
Gobert, GN;Stenzel, DJ;McManus, DP
本文首次用透射电镜对日本血吸虫14.7kDa脂肪酸结合蛋白（Sj-FABPc）进行了定位研究。针对重组Sj-FABPc的多克隆抗体与胶体金标记物组合使用，以确定成虫内蛋白质的分布。Sj-FABPc定位于雄性寄生虫被膜下区域下方的脂滴中。此外，Sj-FABPc存在于雌虫卵黄腺的卵黄液滴中。在雄性或雌性寄生虫的表面或被膜内没有检测到Sj-FABPc。Sj-FABPc在S.日本血吸虫的同源物Sm-FABPc是一种重要的抗-S.还讨论了曼氏疫苗靶分子。
This paper describes the first localization study of the 14.7 kDa fatty acid-binding protein in Schistosoma japonicum (Sj-FABPc) using transmission electron microscopy. A polyclonal antibody raised against recombinant Sj-FABPc was used in combination with a colloidal gold marker to determine the distribution of the protein within adult parasites. Sj-FABPc was localized within lipid droplets below the subtegumental region of the male parasite. Additionally, Sj-FABPc was present in the vitelline droplets of the vitelline glands of female parasites. There were no detectable levels of Sj-FABPc on the surface or within the tegument of male or female parasites. Possible functions of Sj-FABPc within S. japonicum and the relevance of these immunolocalization findings in light of the recent reports that the homologue Sm-FABPc is an important anti-S. mansoni vaccine target molecule are also discussed.