Restoration of osteochondral defects by implanting bilayered poly(lactide-co-glycolide) porous scaffolds in rabbit joints for 12 and 24 weeks
Restoration of osteochondral defects by implanting bilayered poly(lactide-co-glycolide) porous scaffolds in rabbit joints for 12 and 24 weeks
复制标题
双层聚丙交酯乙交酯共聚物多孔支架植入兔关节12周和24周修复骨软骨缺损
DOI：
10.1016/j.jot.2019.04.006
复制
发表时间：
2019-10-01
影响因子：
6.6
通讯作者：
Ding, Jiandong
中科院分区：
文献类型：
--
作者：
Duan, Pingguo;Pan, Zhen;Ding, Jiandong
研究背景：随着人口老龄化和运动损伤的增加，关节损伤的数量也大大增加。组织工程或组织再生是修复关节软骨缺损的重要方法。虽然它最近已经受到了很大的关注，使用双层多孔支架修复软骨和软骨下骨，它是有趣的，以检查在何种程度上由相同种类的生物可降解聚合物聚（丙交酯-共-乙交酯）（PLGA）组成的双层支架可以恢复骨软骨缺损。在此，我们制作了双层PLGA支架，并使用兔模型来检查植入有或没有骨髓间充质干细胞（BMSCs）的多孔支架的功效。方法：在23只家兔的膝关节内侧髁上造成直径4 mm、深5 mm的骨软骨缺损。然后将软骨层中孔径为100 - 200 μ m且骨层中孔径为300-450 μ m的双层PLGA支架（在软骨层中接种或不接种BMSC）移植到每个膝关节的骨软骨缺损中。以相同方式产生的骨软骨缺损未处理以作为对照。在12周和24周后，髁状突收获和分析，使用组织学，免疫组织化学，实时聚合酶链反应，和生物力学测试，以评估疗效的骨软骨修复。结果：没有关节侵蚀，炎症，肿胀，或畸形观察，和所有动物保持全方位的运动。与未处理的空白组相比，植入有或没有细胞的双层支架组表现出更好的表面重建，与周围正常组织相似。含细胞支架修复的新生组织的组织学评分更接近正常组织。虽然新组织的生物力学性能不如正常组织，但在有或无细胞的支架修复的新组织与正常组织的基因水平之间没有发现显著差异。结论：我们的双层PLGA多孔支架支持通过体内组织工程或再生进行长期的骨软骨修复，并且其效果可以在种植同种异体BMSCs的支架下进一步促进。本文的翻译潜力：双层PLGA多孔支架可以促进骨软骨缺损的修复，在骨软骨组织工程中具有应用潜力。(C)2019年，任作家。由爱思唯尔（新加坡）私人有限公司代表华语矫正学会出版。
Background: With the ageing of the population and the increase of sports injuries, the number of joint injuries has increased greatly. Tissue engineering or tissue regeneration is an important method to repair articular cartilage defects. While it has recently been paid much attention to use bilayered porous scaffolds to repair both cartilage and subchondral bone, it is interesting to examine to what extent a bilayer scaffold composed of the same kind of the biodegradable polymer poly(lactide-co-glycolide) (PLGA) can restore an osteochondral defect. Herein, we fabricated bilayered PLGA scaffolds and used a rabbit model to examine the efficacy of implanting the porous scaffolds with or without bone marrow mesenchymal stem cells (BMSCs). The present manuscript reports the regenerative potential up to 24 weeks.Methods: The osteochondral defect, 4 mm in diameter and 5 mm in depth, was created in the medial condyle of each knee in 23 rabbits. The bilayered PLGA scaffolds with a pore size of 100 -200 mu m in the chondral layer and a pore size of 300-450 mu m in the osseous layer, seeded with or without BMSCs in the chondral layer, were then transplanted into the osteochondral defect of each knee. The osteochondral defect created in the same manner was untreated to act as the control. At 12 and 24 weeks postoperatively, condyles were harvested and analyzed using histology, immunohistochemistry, real-time polymerase chain reaction, and biomechanical testing to evaluate the efficacy of osteochondral repair.Results: No joint erosion, inflammation, swelling, or deformity was observed, and all animals maintained a full range of motion. Compared with the untreated blank group, the groups implanting the bilayered scaffolds with or without cells exhibited much better resurfacing, similar to the surrounding normal tissue. The histological scores of neotissues repaired by the scaffold with cells were closer to that of normal tissue. Although the biomechanical properties of neotissues were not as good as the normal tissue, no significant difference was found between the gene levels of neotissues repaired by the scaffold with or without cells and that of the normal tissue. The repair of the osteochondral defect tends to be stable 12 weeks after implantation.Conclusions: Our bilayered PLGA porous scaffold supports long-term osteochondral repair via in vivo tissue engineering or regeneration, and its effect can be further facilitated under the scaffold seeded with allogenic BMSCs. The translational potential of this article: The bilayered PLGA porous scaffold can facilitate the repair of osteochondral defects and has potential for application in osteochondral tissue engineering. (C) 2019 The Authors. Published by Elsevier (Singapore) Pte Ltd on behalf of Chinese Speaking Orthopaedic Society.