Contribution of classical complement activation and IgM to the control of Rickettsia infection.
Contribution of classical complement activation and IgM to the control of Rickettsia infection.
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经典补体激活和IgM在控制立克次体感染中的作用
DOI：
10.1111/mmi.14839
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发表时间：
2021-12
影响因子：
3.6
通讯作者：
Riley SP
中科院分区：
文献类型：
--
作者：
Dahmani M;Cook JH;Zhu JC;Riley SP
致病性立克次体是专性细胞内细菌，是许多危及生命的传染病的病原体。由于这些感染的严重性，必须确定反应性免疫感觉通路，并了解限制立克次体增殖的相关免疫机制。先前的研究表明，哺乳动物补体系统在立克次体感染期间被激活，并有助于对感染的免疫应答。为了进一步确定哺乳动物抗立克次体免疫应答的这一组成部分，我们试图确定立克次体感染期间补体激活的机制。我们采用了一系列的体外和体内感染模型，以研究经典的补体激活途径在立克次体感染过程中的作用。补体活性的消耗或消除表明C1 q和预先存在的IgM都有助于补体激活;因此在立克次体介导的补体激活中涉及经典补体系统。从小鼠中消除经典补体途径增加了对澳洲红蜱感染的易感性，同时增加了多个组织中的细菌负荷和降低了免疫活化标志物。这项研究强调了经典补体途径在抗立克次体免疫中的作用，并暗示常驻立克次体反应性IgM参与了对感染的反应。致病性立克次体是寄生在哺乳动物脉管系统细胞质中的极其危险的细菌。由于这些感染的严重性，必须确定响应性免疫感觉通路并了解限制细菌生长的相关免疫机制。该手稿确定了补体系统的一种成分和预先存在的IgM作为这些细菌免疫识别的关键贡献者。
Pathogenic Rickettsia are obligate intracellular bacteria and the etiologic agents of many life‐threatening infectious diseases. Due to the serious nature of these infections, it is imperative to both identify the responsive immune sensory pathways and understand the associated immune mechanisms that restrict Rickettsia proliferation. Previous studies have demonstrated that the mammalian complement system is both activated during Rickettsia infection and contributes to the immune response to infection. To further define this component of the mammalian anti‐Rickettsia immune response, we sought to identify the mechanism(s) of complement activation during Rickettsia infection. We have employed a series of in vitro and in vivo models of infection to investigate the role of the classical complement activation pathway during Rickettsia infection. Depletion or elimination of complement activity demonstrates that both C1q and pre‐existing IgM contribute to complement activation; thus implicating the classical complement system in Rickettsia‐mediated complement activation. Elimination of the classical complement pathway from mice increases susceptibility to R. australis infection with both increased bacterial loads in multiple tissues and decreased immune activation markers. This study highlights the role of the classical complement pathway in immunity against Rickettsia and implicates resident Rickettsia‐responsive IgM in the response to infection. Pathogenic Rickettsia are extremely dangerous bacteria that parasitize the cytoplasm of the mammalian vasculature. Due to the serious nature of these infections, it is imperative to both identify the responsive immune sensory pathways and understand the associated immune mechanisms that restrict bacterial growth. This manuscript identifies a component of the complement system and pre‐existing IgM as key contributors to immune recognition of these bacteria.
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影响因子：
5.7
作者：
Fish AI;Riley SP;Singh B;Riesbeck K;Martinez JJ
通讯作者：
Martinez JJ
DOI：
10.4049/jimmunol.1100338
发表时间：
2011-12-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Fusakio ME;Mohammed JP;Laumonnier Y;Hoebe K;Köhl J;Mattner J
通讯作者：
Mattner J
影响因子：
3.1
作者：
Bechelli, Jeremy;Smalley, Claire;Fang, Rong
通讯作者：
Fang, Rong
DOI：
10.4049/jimmunol.1700943
发表时间：
2017-11-15
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Baumgarth N
通讯作者：
Baumgarth N
影响因子：
2.9
作者：
Gadjeva, Mihaela G.;Rouseva, Marieta M.;Kojouharova, Mihaela S.
通讯作者：
Kojouharova, Mihaela S.