Antigenic Maps of Influenza A(H3N2) Produced With Human Antisera Obtained After Primary Infection.
Antigenic Maps of Influenza A(H3N2) Produced With Human Antisera Obtained After Primary Infection.
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DOI：
10.1093/infdis/jiv367
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发表时间：
2016-01-01
期刊：
影响因子：
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通讯作者：
Rimmelzwaan GF
中科院分区：
文献类型：
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作者：
Fonville JM;Fraaij PL;de Mutsert G;Wilks SH;van Beek R;Fouchier RA;Rimmelzwaan GF
流感病毒的抗原性表征通常基于针对毒株特异性感染后雪貂抗血清检测的病毒分离株的血凝抑制（HI）测定数据。在此，使用来自原发性甲型流感（H3N2）感染的人的血清学数据进行类似的病毒表征。我们筛选了1995年至2011年从9至24个月大的儿童中收集的血清中的流感病毒抗体，对1989年至2011年分离的23种流感病毒的阳性血清进行了HI试验，并测量了24只雪貂针对同一组病毒的甲型流感（H3N2）抗血清的HI滴度。在17份阳性人血清中，6份具有高应答，显示出预期来自初次感染抗血清的HI模式，而11份血清具有较低、更分散的反应性模式，这不容易解释。基于高应答人HI数据的抗原图谱与使用雪貂数据创建的图谱相似。虽然雪貂和人类抗原图谱的整体结构相似，但病毒位置的局部差异表明人类和雪貂免疫系统可能对病毒的抗原特性有不同的看法。需要进一步研究以确定雪貂和人类数据中血清学模式之间的相似程度。
Antigenic characterization of influenza viruses is typically based on hemagglutination inhibition (HI) assay data for viral isolates tested against strain-specific postinfection ferret antisera. Here, similar virus characterizations were performed using serological data from humans with primary influenza A(H3N2) infection. We screened sera collected between 1995 and 2011 from children between 9 and 24 months of age for influenza virus antibodies, performed HI tests for the positive sera against 23 influenza viruses isolated between 1989 and 2011, and measured HI titers of antisera against influenza A(H3N2) from 24 ferrets against the same panel of viruses. Of the 17 positive human sera, 6 had a high response, showing HI patterns that would be expected from primary infection antisera, while 11 sera had lower, more dispersed patterns of reactivity that are not easily explained. The antigenic map based on the high-response human HI data was similar to the map created using ferret data. Although the overall structure of the ferret and human antigenic maps is similar, local differences in virus positions indicate that the human and ferret immune system might see antigenic properties of viruses differently. Further studies are needed to establish the degree of similarity between serological patterns in ferret and human data.