SIMPLE PROCEDURE FOR ISOLATION OF RAT LIVER MICROSOMES
SIMPLE PROCEDURE FOR ISOLATION OF RAT LIVER MICROSOMES
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DOI：
10.1016/0014-5793(71)80571-9
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发表时间：
1971-01-01
期刊：
影响因子：
3.5
通讯作者：
NARAYAN, KA
中科院分区：
文献类型：
--
作者：
KAMATH, SA;KUMMEROW, FA;NARAYAN, KA
大鼠肝脏的内质网在均质化后经历广泛的碎裂[1]。在1941年早期，内质网的主要成分被认为是微粒体，占肝脏总量的10 -13%[2]。据报道，大鼠肝微粒体具有50-300 nm的可变大小[3]，与其他亚细胞组分（如细胞核和线粒体）相比，需要高离心力将其分离。因此，标准程序需要像超声波仪这样的复杂仪器，同时非常耗时。利用大鼠肝微粒体和大鼠骨骼肌微粒体[4，51]结合钙离子的特性之一，我们在本通讯中报告了一种分离大鼠肝微粒体的相对简单的方法。
Endoplasmic reticulum of rat liver undergoes extensive fragmentation upon homogenization[l]. The major component of endoplasmic reticulum which accounted for lo-13% of total liver was recognized as microsomes in early 194 1 [2]. The rat liver microsomes were reported to have variable size from 50-300 nm [3], necessitating a high centrifugal force to isolate them in contrast to other subcellular fractions like nuclei and mitochondria. The standard procedure, thus requires a sophisticated instrument like an ultracentrifuge and, at the same time, is very time consuming. Taking advantage of one of the characteristic properties of rat liver microsomes and rat skeletal muscle microsomes [4, 51 to bind calcium ions, we report in this communication a relatively simple method for the isolation of rat liver microsomes.