The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion.
The sequence of development of intestinal tissue injury after strangulation ischemia and reperfusion.
复制标题
DOI：
--
复制
发表时间：
1990-05
期刊：
影响因子：
3.8
通讯作者：
P. Park;Ulf Haglund;G. Bulkley;K. Fält
中科院分区：
文献类型：
--
作者：
P. Park;Ulf Haglund;G. Bulkley;K. Fält
已经报道了部分缺血后再灌注时的组织损伤。然而，以前的尝试，以证明一个组成部分的损伤所造成的再灌注后完全缺血已经失败。进行本研究以评估这样的假设，即在这种情况下，由缺血本身引起的组织损伤的程度阻止了再灌注成分的检测。通过产生优先静脉闭塞（绞窄）1至90分钟的静水压钳使大鼠经历近全肠缺血。由盲法检查者在显微镜下评价组织损伤。20分钟或更短的缺血时间不会引起可检测的组织损伤。缺血时间越长，绒毛损伤越严重。然而，无论缺血持续时间如何，再灌注后均未观察到损伤加重。在一个单独的大鼠系列中，采用完全动脉闭塞，没有伴随的静脉充血。持续40至60分钟的这种隔离动脉闭塞之后，再灌注时组织损伤在统计学上显著加重。因此，如果没有伴随的充血，如果缺血性损伤不是太广泛，则全肠缺血可能随后发生再灌注损伤。
Tissue injury at reperfusion has been reported after partial ischemia. However, previous attempts to demonstrate a component of injury caused by reperfusion after total ischemia have failed. This study was performed to evaluate the hypothesis that in such situations the extent of the tissue injury caused by ischemia itself prevented detection of a reperfusion component. Rats were subjected to near-total intestinal ischemia by means of a hydrostatic pressure clamp that produced preferential venous occlusion (strangulation) for periods from 1 to 90 minutes. Tissue injury was evaluated microscopically by a blinded examiner. Ischemic periods of 20 minutes or less did not induce detectable tissue injury. Longer durations of ischemia caused villous injury: the longer the period of ischemia, the more extensive the tissue injury. However, there was no exacerbation of injury seen after reperfusion, regardless of the duration of ischemia. In a separate series of rats, total arterial occlusion was employed without concomitant venous congestion. Such isolation arterial occlusion of 40 to 60 minutes' duration was followed by a statistically significant exacerbation of tissue injury at reperfusion. Thus total intestinal ischemia may be followed by reperfusion injury if there is no concomitant congestion and if ischemic injury is not too extensive.