Simultaneous manipulation of intestinal capacities and nutrient loads in mice.
Simultaneous manipulation of intestinal capacities and nutrient loads in mice.
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同时控制小鼠肠道容量和营养负荷。
DOI：
10.1152/ajpgi.1996.271.6.g969
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Diamond,J
中科院分区：
文献类型：
--
作者：
Hammond,KA;Lam,M;Lloyd,KC;Diamond,J
为了研究小肠容量与负荷的关系，我们同时改变了小鼠的膳食营养负荷和小肠容量。单独的肠横断导致肠质量增加，因为增加了浆膜质量。由于处女小鼠肠切除前具有180%的储备吸收能力和肠切除后再生，切除高达50%的食物摄入量，消化效率，肠道刷状缘葡萄糖摄取率，或除盲肠外的所有器官的测量质量没有影响。肠质量和葡萄糖摄取能力的再生是定量完整的，因为切除后10周的肠质量是类似于未切除小鼠。哺乳期小鼠切除的肠道质量比处女小鼠切除后立即大4倍。盲肠质量增加50%切除泌乳小鼠高食物摄入量，表明营养溢出到远端肠道作为再生的信号。小鼠未能在70%的肠道切除后存活，可能是因为肠道储备吸收能力在手术后立即耗尽，使得再生不可能。
To study the relationship between capacity and load in the small intestine, we simultaneously varied dietary nutrient load and intestinal capacity in mice. Intestinal transection alone caused an increase in intestinal mass, because of increased serosal mass. Because virgin mouse intestine possesses 180% reserve uptake capacity before resection and the intestine regenerates after resection, resection of up to 50% had no effect on food intake, digestive efficiency, intestinal brush-border glucose uptake rate, or mass of all organs measured except the cecum. Regeneration of intestinal mass and glucose uptake capacity was quantitatively complete, because intestinal mass 10 wk after resection was similar to that in unresected mice. Resected intestinal mass in lactating mice was four times larger than that immediately after resection in virgin mice. Cecal mass increased in 50%-resected lactating mice with high food intakes, suggesting nutrient spillage into the distal gut as a signal for regeneration. Mice failed to survive 70% resection of the intestine, possibly because intestinal reserve uptake capacity was exhausted immediately after surgery, making regeneration impossible.