In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria
In Vitro and In Vivo Protein Uptake Studies in Plant Mitochondria
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DOI：
10.1007/978-1-4939-2639-8_5
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发表时间：
2015-01-01
期刊：
影响因子：
--
通讯作者：
Murcha, Monika W.
中科院分区：
文献类型：
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作者：
Duncan, Owen;Carrie, Chris;Murcha, Monika W.
蛋白质摄取到线粒体的研究是研究蛋白质的亚细胞分布和确定位置的分子机制的重要工具。在这里，我们描述了五种技术，允许定量或定性监测蛋白质摄取到线粒体使用在体外和体内的方法。
The study of protein uptake into mitochondria is an important tool for investigating the subcellular distribution of proteins and the molecular mechanisms that determine location. Here we describe five techniques that allow the quantitative or qualitative monitoring of protein uptake into mitochondria using both in vitro and in vivo approaches.