BINDING OF TRANSFER-RNA TO THE RIBOSOMAL A-SITES AND P-SITES PROTECTS 2 DISTINCT SETS OF NUCLEOTIDES IN 16 S RIBOSOMAL-RNA
BINDING OF TRANSFER-RNA TO THE RIBOSOMAL A-SITES AND P-SITES PROTECTS 2 DISTINCT SETS OF NUCLEOTIDES IN 16 S RIBOSOMAL-RNA
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DOI：
10.1016/0022-2836(90)90016-f
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发表时间：
1990-01-05
影响因子：
5.6
通讯作者：
NOLLER, HF
中科院分区：
文献类型：
--
作者：
MOAZED, D;NOLLER, HF
当转运RNA与核糖体结合时，它保护16 S rRNA中的一组特征性碱基免受化学探针的侵害。我们使用了几个标准，在体外核糖体-tRNA复合物的基础上，建设良好的特点，分配这些蛋白质的A或P-位点结合。所有这些方法都得出类似的结论。在A位点，tRNA对G529、G530、A1492和A1493（强）和A1408和G1494（弱）有保护作用。在P位点中，受保护的碱基是G693、A794、C795、G926和G1401（强），以及A532、G966、G1338和G1339（弱）。与对23 S rRNA观察到的相反，阻断EF-Tu的释放。核糖体中的GDP对16 SrRNA中碱基的保护没有可检测到的影响。
Transfer RNA protects a characteristic set of bases in 16S rRNA from chemical probes when it binds to ribosomes. We used several criteria, based on construction of well-characterized in vitro ribosome-tRNA complexes, to assign these proteins to A or P-site binding. All of these approaches lead to similar conclusions. In the A site, tRNA caused protection of G529, G530, A1492 and A1493 (strongly), and A1408 and G1494 (weakly). In the P site, the protected bases are G693, A794, C795, G926 and G1401 (strong), and A532, G966, G1338 and G1339 (weak). In contrast to what is observed for 23S rRNA, blocking the release of EF-Tu .cntdot. GDP from the ribosome by kirromycin has no detectable effect on the protection of bases in 16S rRNA.