EXPRESSION OF A LOW-TEMPERATURE-INDUCED PROTEIN IN BRASSICA-NAPUS
EXPRESSION OF A LOW-TEMPERATURE-INDUCED PROTEIN IN BRASSICA-NAPUS
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DOI：
10.1104/pp.108.2.795
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发表时间：
1995-06-01
期刊：
影响因子：
7.4
通讯作者：
JOHNSONFLANAGAN, AM
中科院分区：
文献类型：
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作者：
BOOTHE, JG;DEBEUS, MD;JOHNSONFLANAGAN, AM
BN28是甘蓝型油菜中一种低温诱导的煮溶蛋白。我们使用针对重组BN28的抗体来检测该蛋白在冷驯化植物中的表达，并研究其与植物抗冻性的关系。BN28蛋白稳态水平的变化似乎比mRNA的变化晚了几天。BN28在低温下暴露约8天后首次在免疫印迹上检测到，此后当植物保持在4℃时，其水平保持稳定。放射性标记研究表明BN28的合成速度相对较低。将植株放回控制温度后，蛋白质水平很快就会下降，在脱暖7天后，几乎检测不到或根本检测不到蛋白质。该蛋白的消失先于抗冻能力的丧失，这表明BN28不参与维持质膜的完整性。BN28主要在叶片中表达，似乎具有低温特异性。定量分析表明，BN28在冷驯化叶片中积累的总蛋白约为82.7 pmol mg（-1）。这一浓度与报道的两个2组晚期胚胎发生丰度样蛋白相似。
BN28 is a low-temperature-induced, boiling-soluble protein in Brassica napus. We used antibodies raised against a recombinant BN28 to examine the expression of this protein in cold-acclimating plants and to investigate its relationship to plant freezing tolerance. Changes in the steady-state levels of BN28 protein appear to lag several days behind those of the mRNA. BN28 is first detected on immunoblots after approximately 8 d of exposure to low temperature, and thereafter levels remain stable while plants are maintained at 4 degrees C. Radiolabeling studies indicate that BN28 is synthesized at a relatively low rate. A decline in protein levels is observed soon after returning plants to control temperatures, and little or no protein can be detected after 7 d of deacclimation. The disappearance of the protein precedes a loss in freezing tolerance, suggesting that BN28 is not involved in maintaining plasma membrane integrity. Expression of BN28 is observed primarily in leaves and appears to be low-temperature specific. Quantitative analysis indicated that BN28 accumulates to approximately 82.7 pmol mg(-1) total protein in cold-acclimated leaves. This concentration is similar to that reported for two group 2 late-embryogenesis-abundant-like proteins.